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采用基于化学发光指示剂的蛋白质免疫印迹法检测弯曲杆菌相关性腹泻患儿尿液标本中的免疫球蛋白A

Detection of immunoglobulin A in urine specimens from children with Campylobacter-associated diarrhea by a chemiluminescent indicator-based western immunoblot assay.

作者信息

Wu S J, Pazzaglia G, Haberberger R L, Oprandy J J, Sieckmann D G, Watts D M, Hayes C

机构信息

Naval Medical Research Institute, Bethesda, Maryland 20889-5055.

出版信息

J Clin Microbiol. 1993 May;31(5):1394-6. doi: 10.1128/jcm.31.5.1394-1396.1993.

DOI:10.1128/jcm.31.5.1394-1396.1993
PMID:8501252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262950/
Abstract

A Western blot (immunoblot) assay was used to detect Campylobacter-specific immunoglobulin A in urine. Acute-phase urine samples from six children with Campylobacter diarrhea had titers ranging from 2 to 8. The highest titer was detected 4 days postonset. Campylobacter-specific immunoglobulin A was undetectable in the paired convalescent-phase specimens and urine samples from three control children.

摘要

采用蛋白质印迹法（免疫印迹法）检测尿液中弯曲杆菌特异性免疫球蛋白A。6名患弯曲杆菌腹泻儿童的急性期尿液样本滴度在2至8之间。发病后4天检测到最高滴度。在配对的恢复期样本以及3名对照儿童的尿液样本中未检测到弯曲杆菌特异性免疫球蛋白A。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26e/262950/551e98ba1413/jcm00017-0391-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26e/262950/037e0b89f580/jcm00017-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26e/262950/551e98ba1413/jcm00017-0391-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26e/262950/037e0b89f580/jcm00017-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26e/262950/551e98ba1413/jcm00017-0391-b.jpg

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本文引用的文献

1
Identification and characterization of Campylobacter jejuni outer membrane proteins.空肠弯曲菌外膜蛋白的鉴定与特性分析
Infect Immun. 1983 Oct;42(1):276-84. doi: 10.1128/iai.42.1.276-284.1983.
2
The identification of outer membrane proteins and flagella of Campylobacter jejuni.空肠弯曲菌外膜蛋白和鞭毛的鉴定
J Gen Microbiol. 1984 May;130(5):1201-8. doi: 10.1099/00221287-130-5-1201.
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Campylobacter jejuni enteritis; a review.空肠弯曲菌肠炎综述
Trop Geogr Med. 1984 Sep;36(3):215-22.
4
Western blot analysis of intestinal secretory immunoglobulin A response to Campylobacter jejuni antigens in patients with naturally acquired Campylobacter enteritis.自然获得性弯曲杆菌肠炎患者肠道对空肠弯曲杆菌抗原分泌型免疫球蛋白A反应的蛋白质印迹分析
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Urine and faecal IgA response during naturally acquired infection with Campylobacter jejuni.空肠弯曲菌自然感染期间的尿液和粪便免疫球蛋白A反应。
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Western blot analysis of the human antibody response to Campylobacter jejuni cellular antigens during gastrointestinal infection.
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Campylobacter diarrhoea and an association of recent disease with asymptomatic shedding in Egyptian children.埃及儿童弯曲杆菌性腹泻及近期疾病与无症状排菌的关联。
Epidemiol Infect. 1991 Feb;106(1):77-82. doi: 10.1017/s0950268800056466.
8
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Infect Immun. 1991 Aug;59(8):2555-9. doi: 10.1128/iai.59.8.2555-2559.1991.