采用基于化学发光指示剂的蛋白质免疫印迹法检测弯曲杆菌相关性腹泻患儿尿液标本中的免疫球蛋白A
Detection of immunoglobulin A in urine specimens from children with Campylobacter-associated diarrhea by a chemiluminescent indicator-based western immunoblot assay.
作者信息
Wu S J, Pazzaglia G, Haberberger R L, Oprandy J J, Sieckmann D G, Watts D M, Hayes C
机构信息
Naval Medical Research Institute, Bethesda, Maryland 20889-5055.
出版信息
J Clin Microbiol. 1993 May;31(5):1394-6. doi: 10.1128/jcm.31.5.1394-1396.1993.
Abstract
A Western blot (immunoblot) assay was used to detect Campylobacter-specific immunoglobulin A in urine. Acute-phase urine samples from six children with Campylobacter diarrhea had titers ranging from 2 to 8. The highest titer was detected 4 days postonset. Campylobacter-specific immunoglobulin A was undetectable in the paired convalescent-phase specimens and urine samples from three control children.
摘要
采用蛋白质印迹法（免疫印迹法）检测尿液中弯曲杆菌特异性免疫球蛋白A。6名患弯曲杆菌腹泻儿童的急性期尿液样本滴度在2至8之间。发病后4天检测到最高滴度。在配对的恢复期样本以及3名对照儿童的尿液样本中未检测到弯曲杆菌特异性免疫球蛋白A。
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