Gray A B, Telford R D, Collins M, Baker M S, Weidemann M J
Division of Biochemistry and Molecular Biology, School of Life Science, Faculty of Science, Australian National University, Canberra.
J Leukoc Biol. 1993 May;53(5):591-7. doi: 10.1002/jlb.53.5.591.
Activation of granulocytes has been associated with normal immune function, inflammation, and exercise-induced muscle damage. The effect of intense interval running on granulocyte activation was examined by use of flow cytometry, monoclonal antibodies, and spectrophotometric techniques. Eight trained males [maximal oxygen uptake VO2max, mean (SD) = 64.4 (3.6) ml/kg/min; age 30.1 (4.8) years] undertook an intense interval exercise (treadmill running) protocol to exhaustion. Subjects completed an average of 16.5 one-minute runs. Granulocyte expression of CR3 (CD11b), receptor for complement component C3bi (6 and 24 h post-test), and Fc gamma RIII (CD16) (24 h post-test) and the plasma concentration of elastase-inhibitor complex (1 h post-test) increased significantly (all P < .05). Subjects (8 of 8) exhibited a post-test decrease at either 1 or 6 h (P < .01) and a 24-h post-test significant increase (7 of 8; P < .05) in granulocyte 90 degrees light scattering (LS). Plasma lactoferrin (Lf) concentration, although increased by 17% at 6 h post-test, was not significantly different from resting values at any sampling point. Changes in plasma Lf and median channel 90 degrees LS were significantly correlated (r = -.43, P = .04), raising the possibility of monitoring exercise-induced granulocyte activation (degranulation) by flow cytometry. Intense interval exercise appears to induce granulocyte activation, as manifested by release of granule proteins and changes in 90 degrees LS and expression of both Fc and complement receptors.
粒细胞的激活与正常免疫功能、炎症以及运动诱导的肌肉损伤有关。通过流式细胞术、单克隆抗体和分光光度技术研究了高强度间歇跑对粒细胞激活的影响。八名受过训练的男性[最大摄氧量VO2max，平均值（标准差）=64.4（3.6）ml/kg/min；年龄30.1（4.8）岁]进行了高强度间歇运动（跑步机跑步）直至力竭。受试者平均完成了16.5次一分钟跑步。粒细胞CR3（CD11b）、补体成分C3bi受体（测试后6小时和24小时）、FcγRIII（CD16）（测试后24小时）的表达以及弹性蛋白酶-抑制剂复合物的血浆浓度（测试后1小时）显著增加（均P<0.05）。所有受试者（8/8）在测试后1小时或6小时出现下降（P<0.01），在测试后24小时粒细胞90度光散射（LS）显著增加（8/7；P<0.05）。血浆乳铁蛋白（Lf）浓度虽然在测试后6小时增加了17%，但在任何采样点与静息值相比均无显著差异。血浆Lf变化与90度LS中值通道显著相关（r=-0.43，P=0.04），增加了通过流式细胞术监测运动诱导的粒细胞激活（脱颗粒）的可能性。高强度间歇运动似乎会诱导粒细胞激活，表现为颗粒蛋白释放、90度LS变化以及Fc和补体受体表达改变。
