Snyder D E, Zarlenga D S, La Rosa G, Pozio E
U.S. Department of Agriculture, Agricultural Research Service, Animal Parasite Research Laboratory, Auburn, Alabama 36831.
J Parasitol. 1993 Jun;79(3):347-52.
Biological, biochemical, and genetic relationships of Trichinella isolates were assessed and compared from 3 species of Illinois fur-bearing mammals. Tongue muscle collected from 1987 through 1989 from 323 raccoons (Procyon lotor), 9 red fox (Vulpes fulva), and 1 coyote (Canis latrans) were digested and Trichinella muscle larval prevalences and mean intensities (larvae/g) determined. The prevalence and mean intensity of tongue muscle-stage larvae were 2.8% and 44.4% and 326 and 2 larvae/g for raccoon and red fox, respectively. The single coyote examined for muscle larvae was negative. Seven of 13 Trichinella isolates (5 raccoon, 2 red fox) were maintained and amplified in mice. Comparative analyses of DNA from larvae of these isolates and from other Trichinella isolates were performed by dot-blot hybridization using specific repetitive DNA probes. DNA from the 7 Illinois sylvatic isolates reacted with the Trichinella T5 isolate DNA probe pUPB-3.7 and did not cross-react with the Trichinella spiralis probe pBP-2. The sylvatic isolates of Trichinella were also analyzed using 27 allozymes. Their allozymic patterns were similar to Trichinella reference strain T5 from Pennsylvania, except for mannose phosphate isomerase. These results suggest that the Illinois wildlife isolates belong to the Trichinella T5 genotype according to the classification system established by the International Trichinella Reference Centre and as defined here by positive hybridization to the pUPB-3.7 probe. Results from Southern blot analyses using rRNA as a probe and allozyme patterns revealed some heterogeneity among geographical isolates of the T5 genotype.
对来自伊利诺伊州3种有毛哺乳动物的旋毛虫分离株的生物学、生物化学和遗传关系进行了评估和比较。采集了1987年至1989年期间323只浣熊（北美浣熊）、9只赤狐和1只郊狼的舌肌进行消化，并测定旋毛虫肌幼虫的感染率和平均强度（幼虫/克）。浣熊和赤狐舌肌阶段幼虫的感染率和平均强度分别为2.8%和44.4%，以及326和2幼虫/克。检查的唯一一只郊狼的肌幼虫为阴性。13株旋毛虫分离株中的7株（5株浣熊、2株赤狐）在小鼠体内进行了保存和扩增。使用特异性重复DNA探针通过斑点杂交对这些分离株的幼虫DNA和其他旋毛虫分离株进行了比较分析。来自伊利诺伊州7株野生分离株的DNA与旋毛虫T5分离株DNA探针pUPB - 3.7发生反应，且不与旋毛虫探针pBP - 2发生交叉反应。还使用27种同工酶对旋毛虫的野生分离株进行了分析。除磷酸甘露糖异构酶外，它们的同工酶模式与宾夕法尼亚州的旋毛虫参考菌株T5相似。这些结果表明，根据国际旋毛虫参考中心建立的分类系统以及此处通过与pUPB - 3.7探针阳性杂交所定义的，伊利诺伊州野生动物分离株属于旋毛虫T5基因型。使用rRNA作为探针的Southern印迹分析结果和同工酶模式揭示了T5基因型地理分离株之间的一些异质性。
