Zhang H
Beijing Tropical Medicine Research Institute.
Zhonghua Liu Xing Bing Xue Za Zhi. 1993 Feb;14(1):49-51.
Dot-ELISA was used with HRP-labelled monoclonal antibodies Kp3 (IgGi) and Kp4-6 (IgM) against antigen of the Karp strain of Rickettsia tsutsugamushi to detect the antigen of R. tsutsugamushi. The positive rate of sera of 56 acute scrub typhus patients and 37 wild rats caught from the countryside of the endemic area as well as 29 batches of chiggers collected from the wild rats were 92.9%, 100% and 89.7%, respectively. No cross reaction was found with antigens of other rickettsia groups such as R. mooseri, R. prowazeki, R.burneti and R. rickettsia. In patients suffering from acute scrub typhus the antigen of R. tsutsugamushi could be detected earlier in the course of illness than the antibody. This method is very sensitive that an amount of antigen of 6.7ng/microliters can be detected. It is easy to perform and the results can be read by naked eye.
采用斑点酶联免疫吸附试验（Dot-ELISA），使用辣根过氧化物酶（HRP）标记的抗恙虫病立克次体卡尔普株抗原的单克隆抗体Kp3（IgG1）和Kp4-6（IgM）检测恙虫病立克次体抗原。56例急性恙虫病患者血清、37只从流行区农村捕获的野鼠血清以及从野鼠身上采集的29批恙螨的阳性率分别为92.9%、100%和89.7%。未发现与莫氏立克次体、普氏立克次体、伯纳特立克次体和立氏立克次体等其他立克次体群抗原发生交叉反应。在急性恙虫病患者中，恙虫病立克次体抗原在病程中比抗体更早被检测到。该方法非常灵敏，能检测到6.7纳克/微升的抗原量。操作简便，结果可肉眼读取。
