Lowichik A, Lanners H N, Lowrie R C, Meiners N E
Department of Tropical Medicine and Parasitology, Tulane University, New Orleans, Louisiana 70115.
J Eukaryot Microbiol. 1993 May-Jun;40(3):287-97. doi: 10.1111/j.1550-7408.1993.tb04918.x.
The sexual life cycle of the hemogregarine Hepatozoon mocassini was studied in Aedes aegypti, an experimental mosquito host, using transmission electron microscopy. Gamonts were observed leaving the host snake erythrocyte as early as 30 min after mosquitoes ingested infected blood, and some gamonts had penetrated the gut epithelial cells by this time. Six hours post-feeding, gamonts were identified within cells of the abdominal fat body. Twenty-four hours post-feeding, gamonts were often entrapped within the peritrophic membrane, but were no longer observed within the gut wall. Parasites pairing up in syzygy and undergoing sexual differentiation were observed within fat cells at this time, and by 48 hours post-feeding, well-developed macro- and microgametocytes as well as microgametes were discernible. Developing zygotes observed 3 days post-feeding were enclosed within a parasitophorous vacuole. By day 6, multinucleate oocysts with crystalloid bodies in the cytoplasm were seen. Sporozoites developing within sporocysts appeared by day 12. Seventeen days post-feeding, mature oocysts with sporocysts containing approximately 16 sporozoites were observed upon dissection of mosquitoes. Large crystalloid bodies no longer bound by rough endoplasmic reticulum were located anterior and posterior to the sporozoite nucleus. Free sporozoites were not observed.
利用透射电子显微镜，在实验性蚊宿主埃及伊蚊中研究了血簇虫莫卡锡肝簇虫的性生活史。早在蚊子摄取感染血液后30分钟，就观察到配子体离开宿主蛇红细胞，此时一些配子体已穿透肠道上皮细胞。喂食后6小时，在腹部脂肪体的细胞内鉴定出配子体。喂食后24小时，配子体常被困在围食膜内，但在肠壁内不再观察到。此时在脂肪细胞内观察到配子体成对结合并进行性分化，到喂食后48小时，可辨认出发育良好的大配子母细胞、小配子母细胞以及小配子。喂食后3天观察到的发育中的合子被包裹在一个寄生泡内。到第6天，可见到细胞质中有晶体状小体的多核卵囊。到第12天，在孢子囊内发育的子孢子出现。喂食后17天，解剖蚊子时观察到含有约16个子孢子的带有孢子囊的成熟卵囊。不再被粗面内质网包围的大晶体状小体位于子孢子核的前后。未观察到游离子孢子。
