Eiden M V, Farrell K, Warsowe J, Mahan L C, Wilson C A
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland 20892.
J Virol. 1993 Jul;67(7):4056-61. doi: 10.1128/JVI.67.7.4056-4061.1993.
A fibroblast cell line (MDTF) derived from the feral mouse Mus dunni is resistant to infection by Moloney murine leukemia virus (Mo-MuLV), an ecotropic murine leukemia virus (E-MuLV) (M. R. Lander and S. K. Chattopadadhyay, J. Virol. 52:695-698, 1984). MDTF cells can be infected by other E-MuLVs such as Friend MuLV and Rauscher MuLV, which have been demonstrated to use the same receptor as Mo-MuLV in NIH 3T3 cells (A. Rein and A. Schultz, Virology 136:144-152, 1984). We have now shown that the block to Mo-MuLV infection of MDTF cells occurs at the level of the envelope-receptor interaction. We have cloned the ecotropic receptor cDNA from MDTF cells (dRec) and compared its sequence with that of the NIH 3T3 cell receptor (mRec). Although the deduced dRec and mRec proteins differ at only four amino acid residues, we demonstrate that these changes account for the resistance of MDTF cells to Mo-MuLV infection. Our findings suggest that retroviruses in the same receptor class can exhibit different host ranges due to single amino acid differences in their cellular receptor.
一种源自野生小家鼠（Mus dunni）的成纤维细胞系（MDTF）对莫洛尼鼠白血病病毒（Mo-MuLV）具有抗性，莫洛尼鼠白血病病毒是一种亲嗜性鼠白血病病毒（E-MuLV）（M. R. 兰德和S. K. 恰托帕达德哈亚，《病毒学杂志》52:695 - 698，1984年）。MDTF细胞可被其他E-MuLV感染，如弗氏鼠白血病病毒和劳氏鼠白血病病毒，在NIH 3T3细胞中已证明它们与Mo-MuLV使用相同的受体（A. 赖因和A. 舒尔茨，《病毒学》136:144 - 152，1984年）。我们现已表明，MDTF细胞对Mo-MuLV感染的阻断发生在包膜 - 受体相互作用水平。我们已从MDTF细胞中克隆出亲嗜性受体cDNA（dRec），并将其序列与NIH 3T3细胞受体（mRec）的序列进行了比较。尽管推导的dRec和mRec蛋白仅在四个氨基酸残基处不同，但我们证明这些变化导致了MDTF细胞对Mo-MuLV感染的抗性。我们的研究结果表明，同一受体类别的逆转录病毒由于其细胞受体中的单个氨基酸差异可表现出不同的宿主范围。
