Beklemishev A B, Nazarova L M, Filimonov N G, Blinov V M, Grinev A A, Chuvakova Z K, Kim E V, Mukazhanova G N
Mol Gen Mikrobiol Virusol. 1993 Jan-Feb(1):24-7.
The full-length copy of the hemagglutinin gene RNA of the influenza virus A/Alma Ata/1417/84 (Hsw1 N1-serovariant) has been synthesized and cloned on Escherichia coli plasmid pBR327. The complete nucleotide sequence of the cloned DNA copy was determined by the Maxam-Gilbert procedure. The predicted amino acid, sequence of HA1 hemagglutinin subunit was compared with the sequences of HA1 subunits from other H1N1-subtype influenza virus strains. It has been found that the structure of the HA1-subunit of the studied strain is most similar to the structure of the identical region of the A/New Jersey/18/76 hemagglutinin.
甲型流感病毒A/阿拉木图/1417/84(Hsw1 N1血清变种)血凝素基因RNA的全长拷贝已被合成并克隆到大肠杆菌质粒pBR327上。通过马克萨姆-吉尔伯特法测定了克隆DNA拷贝的完整核苷酸序列。将预测的HA1血凝素亚基的氨基酸序列与其他H1N1亚型流感病毒株的HA1亚基序列进行了比较。已发现所研究毒株的HA1亚基结构与A/新泽西/18/76血凝素相同区域的结构最为相似。