Uvarov V D, Shepelev A P, Poliakov V M
Vopr Med Khim. 1993 Mar-Apr;39(2):58-62.
A lipopolysaccharide preparation obtained from Klebsiella pneumonia was shown to affect primarily the peritoneal macrophages after intraperitoneal administration. Transition of the macrophages to a new metabolic state in response to opsonized zymosan was responsible for a distinct increase in the rate of luminol-dependent chemoluminescence. At the same time, the macrophages produced chemiluminescence was only slightly increased in the presence of Ca2+ ionophore A23187. These dissimilar alterations observed suggest that the primary reaction of macrophages in response to the lipopolysaccharide occurred with the increase in content of intracellular free calcium. Administration of finoptin (calcium influx inhibitor) into suspension of macrophages caused a decrease in the rate of chemiluminescence response.
从肺炎克雷伯菌中提取的一种脂多糖制剂经腹腔注射后，主要影响腹膜巨噬细胞。巨噬细胞因调理酵母聚糖而转变为新的代谢状态，导致鲁米诺依赖性化学发光速率显著增加。同时，在存在Ca2+离子载体A23187的情况下，巨噬细胞产生的化学发光仅略有增加。观察到的这些不同变化表明，巨噬细胞对脂多糖的主要反应是细胞内游离钙含量增加。向巨噬细胞悬液中加入芬普替林（钙内流抑制剂）会导致化学发光反应速率降低。
