[用于检测鸡和小鼠抗新城疫病毒抗体的酶免疫测定法的开发]
[Development of an enzyme immunoassay for the detection of antibodies from hens and mice against Newcastle disease virus].
作者信息
Minning P, Hlinak A, Klaczinski K
机构信息
Institut für Virologie und Geflügelkrankheiten, Veterinärmedizinischen Fakultät, Humboldt-Universität zu Berlin.
出版信息
Berl Munch Tierarztl Wochenschr. 1993 May;106(5):149-53.
The development of an enzyme-immuno-assay (EIA) for detection of avian and mouse antibodies against the Newcastle-Disease Virus is shown. The antigen preparation was the centre of the experiments. The basic assay programme and selected test steps were optimized. The established assay is part of the EIA-control system for a SPF (specific-pathogen-free)-flock and also the essential screening method for the production of anti-NDV-monoclonal antibodies.
本文展示了一种用于检测禽类和小鼠抗新城疫病毒抗体的酶免疫测定法（EIA）的开发过程。抗原制备是实验的核心。对基本测定程序和选定的测试步骤进行了优化。所建立的测定法是无特定病原体（SPF）鸡群EIA控制系统的一部分，也是生产抗新城疫病毒单克隆抗体的重要筛选方法。
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