Adamis A P, Shima D T, Yeo K T, Yeo T K, Brown L F, Berse B, D'Amore P A, Folkman J
Department of Surgery, Childrens Hospital, Boston, Massachusetts 02115.
Biochem Biophys Res Commun. 1993 Jun 15;193(2):631-8. doi: 10.1006/bbrc.1993.1671.
The presence of the secreted angiogenic molecule, vascular permeability factor/vascular endothelial growth factor, was investigated in human retinal pigment epithelial cells. Northern and in situ hybridization analysis of cultured cells identified vascular permeability factor/vascular endothelial growth factor mRNA. Western analysis of cell lysates identified a 42 kD protein that comigrated with human recombinant vascular permeability factor/vascular endothelial growth factor. Immunoassay measurements detected vascular permeability factor/vascular endothelial growth factor protein in cell lysates and conditioned media in vitro and in cell lysates isolated directly from post-mortem eyes. These data demonstrate that human retinal pigment epithelial cells can synthesize the secreted angiogenic peptide vascular permeability factor/vascular endothelial growth factor in vitro and in situ. The production and secretion of this factor by human retinal pigment epithelial cells may be important in the pathogenesis of ocular neovascularization.
研究了分泌性血管生成分子血管通透性因子/血管内皮生长因子在人视网膜色素上皮细胞中的存在情况。对培养细胞进行的Northern和原位杂交分析鉴定出了血管通透性因子/血管内皮生长因子mRNA。对细胞裂解物进行的Western分析鉴定出一种与重组人血管通透性因子/血管内皮生长因子迁移率相同的42 kD蛋白。免疫测定检测到体外细胞裂解物和条件培养基以及直接从死后眼睛分离的细胞裂解物中存在血管通透性因子/血管内皮生长因子蛋白。这些数据表明，人视网膜色素上皮细胞在体外和原位均可合成分泌性血管生成肽血管通透性因子/血管内皮生长因子。人视网膜色素上皮细胞产生和分泌该因子可能在眼部新生血管形成的发病机制中起重要作用。
