[用植物血凝素刺激外周血淋巴细胞产生乙型肝炎病毒]
[Production of hepatitis B virus from peripheral blood lymphocytes stimulated with phytohemagglutinin].
作者信息
Sarría L, Gallego L, de las Heras B, Basaras M, Cisterna R
机构信息
Departamento de Microbiología e Inmunología, Universidad del País Vasco.
出版信息
Enferm Infecc Microbiol Clin. 1993 Apr;11(4):187-9.
BACKGROUND
It was studied the production of HBV from lymphocytes from peripheral blood induced with a mitogen agent as phytohemagglutinin (PHA), in patients infected by HIV.
METHODS
The methodology developed included the culture of peripheral blood mononuclear cells and its induction to proliferate with PHA, in order to study the viral activity in the supernatants of the cultures by detection of HIV-Ag (ELISA), HBsAg (ELISA) and DNA-HBV (Dot-blot).
RESULTS
Production of HBV in 23 out of the 42 patients included in this study, corresponding to 21 anti-HIV positive and 2 anti-HIV negative.
CONCLUSIONS
Induction to proliferate lymphocytes with a mitogen agent as PHA may represent an alternative way of culture to study interactions between HIV and HBV.
背景
研究了在感染人类免疫缺陷病毒（HIV）的患者中，用植物血凝素（PHA）等促有丝分裂剂诱导外周血淋巴细胞产生乙肝病毒（HBV）的情况。
方法
所开发的方法包括外周血单个核细胞的培养及其用PHA诱导增殖，以便通过检测HIV抗原（酶联免疫吸附测定法）、乙肝表面抗原（酶联免疫吸附测定法）和乙肝病毒DNA（斑点印迹法）来研究培养上清液中的病毒活性。
结果
本研究纳入的42例患者中有23例产生了HBV，其中21例抗HIV阳性，2例抗HIV阴性。
结论
用PHA等促有丝分裂剂诱导淋巴细胞增殖可能是一种用于研究HIV与HBV之间相互作用的替代性培养方法。
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