Onishi T, Suzuki S, Horie M, Hashimoto M, Kajikawa T, Ohishi I, Ejima H
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
J Vet Med Sci. 1993 Apr;55(2):203-6. doi: 10.1292/jvms.55.203.
The serum hemolytic activity of Babesia gibsoni-infected dogs varied when assayed with nonself red blood cells from different dogs, whereas it did not vary when assayed with red blood cells, irrespective of self or nonself, from a particular dog throughout the experiment. The variety in activity determined with nonself red blood cells was not related to the type of red blood cell by DEA, D and J systems. Serum hemolytic activity with self red blood cells was different in the course of infection from that with nonself red blood cells, especially in the late stage of infection, when the activity with self red blood cells decreased more rapidly than that with nonself red blood cells. The results indicate that the serum hemolytic activity of B. gibsoni-infected dogs determined with self red blood cells probably reflects the in vivo activity, suggesting that the rapid decrease in activity in the late stage of infection is a way of acquired resistances for the host to recover from hemolytic anemia in the infection. The facts that the hemolytic activity increased by heating the serum at 56 degrees C, that the osmotic fragility of red blood cells remained almost on the same during the course of infection and that Coombs' test for red blood cells of the infected animal was negative suggest that the immune-mediated hemolytic anemia is not a possible mechanism for the progressive and severe anemia in B. gibsoni-infection. The present results support the previous notion that the increased serum hemolytic activity is at least one of the causes of anemia in canine B. gibsoni-infection.
用来自不同犬只的非自身红细胞检测时，感染吉氏巴贝斯虫犬的血清溶血活性有所不同；而在整个实验过程中，用来自某一特定犬只的自身或非自身红细胞检测时，其血清溶血活性并无变化。用非自身红细胞测定的活性差异与DEA、D和J系统的红细胞类型无关。感染过程中，自身红细胞的血清溶血活性与非自身红细胞的血清溶血活性不同，尤其是在感染后期，自身红细胞的血清溶血活性比非自身红细胞的血清溶血活性下降得更快。结果表明，用自身红细胞测定的感染吉氏巴贝斯虫犬的血清溶血活性可能反映了体内活性，这表明感染后期活性的快速下降是宿主从溶血性贫血中恢复的一种获得性抗性方式。血清在56℃加热后溶血活性增加、感染过程中红细胞的渗透脆性几乎保持不变以及对感染动物的红细胞进行库姆斯试验呈阴性，这些事实表明免疫介导的溶血性贫血不是吉氏巴贝斯虫感染中进行性严重贫血的可能机制。目前的结果支持了先前的观点，即血清溶血活性增加至少是犬类吉氏巴贝斯虫感染贫血的原因之一。
