Jacobs B, Batal H A, Lynch B, Ojemann G, Ojemann L M, Scheibel A B
Reed Neurological Research Center, UCLA School of Medicine 90024-1769.
Brain Lang. 1993 Apr;44(3):239-53. doi: 10.1006/brln.1993.1016.
The present case study documents an unprecedented opportunity for correlative investigation of brain structure and function by quantitatively investigating the basilar dendritic systems of supragranular pyramidal cells in several cortical areas from a subject who had undergone electrical stimulation mapping 2 years prior to death. Electrical stimulation mapping results provided valuable functional information about the cortical areas removed for postmortem histological analysis. Morphometric analyses distinguished between proximal (first, second, and third order) and ontogenetically later developing distal (fourth order and above) basilar dendritic branches. In general, perisylvian language association stimulation sites (classical Wernicke's and Broca's areas) were characterized by different dendritic patterns than motor strip sites. In primary motor strip tissue blocks, proximal segments were longer than distal segments. In "higher order" elaborative cortical zones, distal segments were longer than proximal segments. Proximal segments outnumbered distal segments in primary motor zones, but the numerical difference between proximal and distal segments was reduced in the association areas. Finally, fourth order segments had significantly more dendritic spines than third order segments in all sites. These dendritic findings suggest a somewhat later ontogenetic development in classical Broca's and Wernicke's areas than in primary motor cortex.
本病例研究记录了一个前所未有的机会，可通过对一名在死亡前2年接受过电刺激图谱检查的受试者的多个皮质区域的颗粒上层锥体细胞的基底树突系统进行定量研究，来关联研究大脑结构与功能。电刺激图谱结果为死后组织学分析所切除的皮质区域提供了有价值的功能信息。形态计量分析区分了近端（第一、第二和第三级）和个体发育后期发育的远端（第四级及以上）基底树突分支。一般来说，颞叶周围语言联合刺激部位（经典的韦尼克区和布洛卡区）的树突模式与运动带部位不同。在初级运动带组织块中，近端节段比远端节段长。在“高级”精细皮质区，远端节段比近端节段长。在初级运动区，近端节段数量多于远端节段，但在联合区近端和远端节段的数量差异减小。最后，在所有部位，第四级节段的树突棘明显多于第三级节段。这些树突研究结果表明，经典布洛卡区和韦尼克区的个体发育比初级运动皮质稍晚。
