Newton I, Charbord P, Schaal J P, Herve P
INSERM/CRTS, Besançon, France.
Exp Hematol. 1993 May;21(5):671-4.
It has been shown that cord blood collected at birth can be used to successfully engraft a human lymphocyte antigen (HLA)-matched sibling suffering from a malignant disease. It has been further suggested that this source of cells may be used in unrelated but HLA-compatible patients. These wide indications would imply the establishment of cord blood banks comprising 10(5) or more samples. In this report we show that it is possible to fractionate and freeze cord blood samples without major loss in granulomonocytic or erythroblastic progenitors (CFU-GM and BFU-E). Density separation should be carried out using Percoll of density 1.080. Separated samples should be frozen and thawed in the presence of DNase I. This procedure should allow the storage of approximately 10 mL samples in cryotubes containing a number of CFU-GM and BFU-E sufficient to engraft a patient weighing less than 30 kg. These data provide a rationale for establishing cord blood banks.
已表明出生时采集的脐带血可成功移植给患有恶性疾病且人类淋巴细胞抗原（HLA）匹配的同胞。进一步有人提出，这种细胞来源可用于无血缘关系但HLA相容的患者。这些广泛的适应证意味着要建立包含10⁵或更多样本的脐带血库。在本报告中，我们表明可以对脐带血样本进行分离和冷冻，而粒单核细胞或成红细胞祖细胞（CFU-GM和BFU-E）不会有重大损失。密度分离应使用密度为1.080的Percoll进行。分离的样本应在DNase I存在的情况下冷冻和解冻。此程序应允许在含有足以移植体重小于30 kg患者的一定数量CFU-GM和BFU-E的冷冻管中储存约10 mL样本。这些数据为建立脐带血库提供了理论依据。
