Abei M, Tanaka N, Matsumoto H, Matsuzaki Y, Osuga T
Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
Gastrointest Endosc. 1993 May-Jun;39(3):410-2. doi: 10.1016/s0016-5107(93)70117-0.
Intravenous injection of indocyanine green improves the laparoscopic diagnosis of liver disease. To clarify the basis for the laparoscopic appearance of indocyanine green-colored liver, local indocyanine green coloration was compared with the hepatic ligandin in the corresponding subcapsular tissue. Two liver biopsy specimens were obtained from each of 13 patients with chronic hepatitis, and the ligandin was stained by an immunohistochemical method. In cases showing homogeneous indocyanine green coloration, the ligandin in the two biopsy specimens was similarly stained. On the other hand, in cases showing inhomogeneous coloration of indocyanine green, biopsy specimens from the well-colored area showed focal regeneration and were rich in ligandin, whereas biopsy specimens from less-colored lesions showed fibrosis, necrosis, or severe inflammation, and the ligandin was poorly stained. These results suggest that the characteristic laparoscopic appearance of the indocyanine green-colored liver reflects the changes of intrahepatic ligandin distribution associated with pathologic changes in the liver of patients with chronic hepatitis.
静脉注射吲哚菁绿可改善肝脏疾病的腹腔镜诊断。为阐明腹腔镜下吲哚菁绿染色肝脏外观的基础，将局部吲哚菁绿染色与相应肝包膜下组织中的肝配蛋白进行了比较。从13例慢性肝炎患者中各获取两份肝活检标本，采用免疫组织化学方法对肝配蛋白进行染色。在显示均匀吲哚菁绿染色的病例中，两份活检标本中的肝配蛋白染色相似。另一方面，在显示吲哚菁绿染色不均匀的病例中，染色良好区域的活检标本显示局灶性再生且富含肝配蛋白，而染色较差病变的活检标本显示纤维化、坏死或严重炎症，且肝配蛋白染色不佳。这些结果表明，吲哚菁绿染色肝脏的特征性腹腔镜外观反映了慢性肝炎患者肝脏病理变化相关的肝内肝配蛋白分布变化。
