酿酒酵母中质粒携带的反向重复序列的不稳定性。
Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae.
作者信息
Henderson S T, Petes T D
机构信息
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
出版信息
Genetics. 1993 May;134(1):57-62. doi: 10.1093/genetics/134.1.57.
Abstract
Inverted repeated DNA sequences are common in both prokaryotes and eukaryotes. We found that a plasmid-borne 94 base-pair inverted repeat (a perfect palindrome of 47 bp) containing a poly GT sequence is unstable in S. cerevisiae, with a minimal deletion frequency of about 10(-4)/mitotic division. Ten independent deletions had identical end points. Sequence analysis indicated that all deletions were the result of a DNA polymerase slippage event (or a recombination event) involving a 5-bp repeat (5' CGACG 3') that flanked the inverted repeat. The deletion rate and the types of deletions were unaffected by the rad52 mutation. Strains with the pms1 mutation had a 10-fold elevated frequency of instability of the inverted repeat. The types of sequence alterations observed in the pms1 background, however, were different than those seen in either the wild-type or rad52 genetic backgrounds.
摘要
反向重复DNA序列在原核生物和真核生物中都很常见。我们发现，一个携带质粒的94个碱基对的反向重复序列（一个47 bp的完美回文序列），包含一个多聚GT序列，在酿酒酵母中不稳定，最小缺失频率约为10^(-4)/有丝分裂。十个独立的缺失具有相同的终点。序列分析表明，所有缺失都是涉及反向重复序列两侧一个5 bp重复序列（5' CGACG 3'）的DNA聚合酶滑动事件（或重组事件）的结果。缺失率和缺失类型不受rad52突变的影响。具有pms1突变的菌株反向重复序列的不稳定频率提高了10倍。然而，在pms1背景中观察到的序列改变类型与野生型或rad52遗传背景中看到的不同。
相似文献
1
Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae.酿酒酵母中质粒携带的反向重复序列的不稳定性。
Genetics. 1993 May;134(1):57-62. doi: 10.1093/genetics/134.1.57.
2
The prevention of repeat-associated deletions in Saccharomyces cerevisiae by mismatch repair depends on size and origin of deletions.酿酒酵母中错配修复对重复相关缺失的预防取决于缺失的大小和起源。
Genetics. 1996 Aug;143(4):1579-87. doi: 10.1093/genetics/143.4.1579.
3
Replication slippage between distant short repeats in Saccharomyces cerevisiae depends on the direction of replication and the RAD50 and RAD52 genes.酿酒酵母中远距离短重复序列之间的复制滑移取决于复制方向以及RAD50和RAD52基因。
Mol Cell Biol. 1995 Oct;15(10):5607-17. doi: 10.1128/MCB.15.10.5607.
4
Formation of large palindromic DNA by homologous recombination of short inverted repeat sequences in Saccharomyces cerevisiae.酿酒酵母中短反向重复序列通过同源重组形成大的回文DNA。
Genetics. 2002 Jul;161(3):1065-75. doi: 10.1093/genetics/161.3.1065.
5
Mutations in POL1 increase the mitotic instability of tandem inverted repeats in Saccharomyces cerevisiae.POL1基因的突变会增加酿酒酵母中串联反向重复序列的有丝分裂不稳定性。
Genetics. 1993 May;134(1):43-56. doi: 10.1093/genetics/134.1.43.
6
Altered replication and inverted repeats induce mismatch repair-independent recombination between highly diverged DNAs in yeast.复制改变和反向重复序列诱导酵母中高度分化的DNA之间发生不依赖错配修复的重组。
Mol Cell Biol. 1997 Feb;17(2):1027-36. doi: 10.1128/MCB.17.2.1027.
7
Stabilization of microsatellite sequences by variant repeats in the yeast Saccharomyces cerevisiae.酿酒酵母中变异重复序列对微卫星序列的稳定作用。
Genetics. 1997 Jun;146(2):491-8. doi: 10.1093/genetics/146.2.491.
8
Factors affecting inverted repeat stimulation of recombination and deletion in Saccharomyces cerevisiae.影响酿酒酵母中重组和缺失的反向重复序列刺激的因素。
Genetics. 1998 Apr;148(4):1507-24. doi: 10.1093/genetics/148.4.1507.
9
Plasmid recombination in a rad52 mutant of Saccharomyces cerevisiae.酿酒酵母rad52突变体中的质粒重组。
Genetics. 1992 Jun;131(2):261-76. doi: 10.1093/genetics/131.2.261.
10
Role of reciprocal exchange, one-ended invasion crossover and single-strand annealing on inverted and direct repeat recombination in yeast: different requirements for the RAD1, RAD10, and RAD52 genes.相互交换、单向侵入交叉和单链退火在酵母中反向和正向重复重组中的作用：对RAD1、RAD10和RAD52基因的不同需求。
Genetics. 1995 Jan;139(1):109-23. doi: 10.1093/genetics/139.1.109.
引用本文的文献
1
RPA and Rad27 limit templated and inverted insertions at DNA breaks.RPA和Rad27限制DNA断裂处的模板化插入和反向插入。
Nucleic Acids Res. 2025 Jan 7;53(1). doi: 10.1093/nar/gkae1159.
2
Pathways and Mechanisms that Prevent Genome Instability in .预防……基因组不稳定的途径和机制
Genetics. 2017 Jul;206(3):1187-1225. doi: 10.1534/genetics.112.145805.
3
A 160-bp palindrome is a Rad50.Rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe.一个160碱基对的回文序列是粟酒裂殖酵母中依赖Rad50.Rad32的有丝分裂重组热点。
Genetics. 2002 May;161(1):461-8. doi: 10.1093/genetics/161.1.461.
4
Inverted repeats as genetic elements for promoting DNA inverted duplication: implications in gene amplification.作为促进DNA反向复制的遗传元件的反向重复序列：对基因扩增的影响
Nucleic Acids Res. 2001 Sep 1;29(17):3529-38. doi: 10.1093/nar/29.17.3529.
5
AT-rich palindromes mediate the constitutional t(11;22) translocation.富含AT的回文序列介导了先天性t(11;22)易位。
Am J Hum Genet. 2001 Jan;68(1):1-13. doi: 10.1086/316952. Epub 2000 Nov 28.
6
Inverted Alu repeats unstable in yeast are excluded from the human genome.在酵母中不稳定的反向Alu重复序列被排除在人类基因组之外。
EMBO J. 2000 Jul 17;19(14):3822-30. doi: 10.1093/emboj/19.14.3822.
7
Long inverted repeats are an at-risk motif for recombination in mammalian cells.长反向重复序列是哺乳动物细胞中发生重组的风险基序。
Genetics. 1999 Dec;153(4):1873-83. doi: 10.1093/genetics/153.4.1873.
8
Arabidopsis PAI gene arrangements, cytosine methylation and expression.拟南芥PAI基因排列、胞嘧啶甲基化与表达
Genetics. 1999 Sep;153(1):401-13. doi: 10.1093/genetics/153.1.401.
9
The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA.大肠杆菌的SbcCD核酸酶是一种能切割发夹DNA的染色体结构维持（SMC）家族蛋白。
Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7969-74. doi: 10.1073/pnas.95.14.7969.
10
Factors affecting inverted repeat stimulation of recombination and deletion in Saccharomyces cerevisiae.影响酿酒酵母中重组和缺失的反向重复序列刺激的因素。
Genetics. 1998 Apr;148(4):1507-24. doi: 10.1093/genetics/148.4.1507.
本文引用的文献
1
Mutations of Bacteria from Virus Sensitivity to Virus Resistance.细菌从对病毒敏感到对病毒抗性的突变。
Genetics. 1943 Nov;28(6):491-511. doi: 10.1093/genetics/28.6.491.
2
Structural intermediates of deletion mutagenesis: a role for palindromic DNA.缺失诱变的结构中间体：回文DNA的作用
Proc Natl Acad Sci U S A. 1984 Jan;81(2):512-6. doi: 10.1073/pnas.81.2.512.
3
Slow cruciform transitions in palindromic DNA.回文DNA中的缓慢十字形转变。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5545-9. doi: 10.1073/pnas.80.18.5545.
4
Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast.构建并使用在酵母中表达的与lacZ（β-半乳糖苷酶）的基因融合体。
Methods Enzymol. 1983;101:167-80. doi: 10.1016/0076-6879(83)01012-5.
5
On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions.关于自发缺失的形成：短序列同源性在大缺失产生中的重要性。
Cell. 1982 Jun;29(2):319-28. doi: 10.1016/0092-8674(82)90148-9.
6
The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.反向重复序列作为超螺旋DNA分子中一种可识别的结构特征。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6468-72. doi: 10.1073/pnas.77.11.6468.
7
Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2.酿酒酵母中的减数分裂基因转换突变体。I. pms1-1和pms1-2的分离与鉴定。
Genetics. 1985 Aug;110(4):609-46. doi: 10.1093/genetics/110.4.609.
8
A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.从酵母中制备十分钟的DNA可有效释放自主质粒用于转化大肠杆菌。
Gene. 1987;57(2-3):267-72. doi: 10.1016/0378-1119(87)90131-4.
9
Mechanisms of spontaneous mutagenesis: an analysis of the spectrum of spontaneous mutation in the Escherichia coli lacI gene.自发诱变机制：大肠杆菌lacI基因自发突变谱的分析
J Mol Biol. 1986 May 20;189(2):273-84. doi: 10.1016/0022-2836(86)90509-7.
10
Spontaneous deletion formation at the aprt locus of hamster cells: the presence of short sequence homologies and dyad symmetries at deletion termini.仓鼠细胞aprt基因座处的自发缺失形成：缺失末端存在短序列同源性和二重对称。
EMBO J. 1986 Jun;5(6):1199-204. doi: 10.1002/j.1460-2075.1986.tb04347.x.
Zotero 插件