Petes T D, Greenwell P W, Dominska M
Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA. tompetes@e mail.unc.edu
Genetics. 1997 Jun;146(2):491-8. doi: 10.1093/genetics/146.2.491.
We examined the effect of a single variant repeat on the stability of a 51-base pair (bp) microsatellite (poly GT). We found that the insertion stabilizes the microsatellite about fivefold in wild-type strains. The stabilizing effect of the variant base was also observed in strains with mutations in the DNA mismatch repair genes pms1, msh2 and msh3, indicating that this effect does not require a functional DNA mismatch repair system. Most of the microsatellite alterations in the pms1, msh2 and msh3 strains were additions or deletions of single GT repeats, but about half of the alterations in the wild-type and msh6 strains were large (> 8 bp) deletions or additions.
我们研究了单个变异重复对一个51个碱基对（bp）微卫星（多聚GT）稳定性的影响。我们发现，在野生型菌株中，该插入使微卫星的稳定性提高了约五倍。在DNA错配修复基因pms1、msh2和msh3发生突变的菌株中也观察到了变异碱基的稳定作用，这表明这种作用不需要功能性的DNA错配修复系统。pms1、msh2和msh3菌株中的大多数微卫星改变是单个GT重复的添加或缺失，但野生型和msh6菌株中约一半的改变是大的（> 8 bp）缺失或添加。
