影响酿酒酵母中重组和缺失的反向重复序列刺激的因素。
Factors affecting inverted repeat stimulation of recombination and deletion in Saccharomyces cerevisiae.
作者信息
Lobachev K S, Shor B M, Tran H T, Taylor W, Keen J D, Resnick M A, Gordenin D A
机构信息
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
出版信息
Genetics. 1998 Apr;148(4):1507-24. doi: 10.1093/genetics/148.4.1507.
Abstract
Inverted DNA repeats are an at-risk motif for genetic instability that can induce both deletions and recombination in yeast. We investigated the role of the length of inverted repeats and size of the DNA separating the repeats for deletion and recombination. Stimulation of both deletion and recombination was directly related to the size of inverted repeats and inversely related to the size of intervening spacers. A perfect palindrome, formed by two 1.0-kb URA3-inverted repeats, increased intra- and interchromosomal recombination in the adjacent region 2,400-fold and 17,000-fold, respectively. The presence of a strong origin of replication in the spacer reduced both rates of deletion and recombination. These results support a model in which the stimulation of deletion and recombination by inverted repeats is initiated by a secondary structure formed between single-stranded DNA of inverted repeats during replication.
摘要
反向DNA重复序列是导致遗传不稳定的一个风险基序，它能在酵母中诱导缺失和重组。我们研究了反向重复序列的长度以及分隔重复序列的DNA大小对缺失和重组的作用。缺失和重组的刺激与反向重复序列的大小直接相关，与间隔序列的大小呈负相关。由两个1.0 kb URA3反向重复序列形成的完美回文结构，分别使相邻区域的染色体内和染色体间重组增加了2400倍和17000倍。间隔序列中存在强复制起点会降低缺失和重组的速率。这些结果支持了一个模型，即反向重复序列对缺失和重组的刺激是由复制过程中反向重复序列的单链DNA之间形成的二级结构引发的。
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