日本甲状腺乳头状癌中ret和trk原癌基因重排的低频率
Low frequency of rearrangements of the ret and trk proto-oncogenes in Japanese thyroid papillary carcinomas.
作者信息
Wajjwalku W, Nakamura S, Hasegawa Y, Miyazaki K, Satoh Y, Funahashi H, Matsuyama M, Takahashi M
机构信息
Department of Pathology, Nagoya University School of Medicine.
出版信息
Jpn J Cancer Res. 1992 Jul;83(7):671-5. doi: 10.1111/j.1349-7006.1992.tb01963.x.
We investigated the frequency of rearrangements of the ret and trk proto-oncogenes in Japanese thyroid tumors. DNAs from 38 thyroid papillary carcinomas and 14 follicular adenomas were analyzed by Southern blotting. Rearrangements of the ret and trk proto-oncogenes were detected in one and two papillary carcinomas, respectively, but not in follicular adenomas. Analysis by a reverse transcriptase-polymerase chain reaction method showed that the ret rearrangement-positive tumor contained the PTC/retTPC chimeric transcript, which was reported to be found specifically in thyroid tumors and adenomatous goiter. We also found that rearranged mRNA of the trk proto-oncogene was expressed at high levels in one of two trk rearrangement-positive tumors. Our results indicated that the frequency of rearrangements of these proto-oncogenes in Japanese papillary carcinomas was much lower than that in Italian patients.
我们研究了日本甲状腺肿瘤中ret和trk原癌基因重排的频率。通过Southern印迹法分析了38例甲状腺乳头状癌和14例滤泡性腺瘤的DNA。ret和trk原癌基因重排在1例和2例乳头状癌中分别被检测到，但在滤泡性腺瘤中未检测到。逆转录酶-聚合酶链反应方法分析显示，ret重排阳性肿瘤含有PTC/retTPC嵌合转录本，据报道该转录本在甲状腺肿瘤和腺瘤性甲状腺肿中特异性存在。我们还发现，trk原癌基因的重排mRNA在2例trk重排阳性肿瘤中的1例中高表达。我们的结果表明，这些原癌基因在日本乳头状癌中的重排频率远低于意大利患者。
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