Nishibe Y, Inoue Y K
Institute for Virus Research, Kyoto University, Japan.
J Virol Methods. 1993 May;42(2-3):345-9. doi: 10.1016/0166-0934(93)90045-s.
Ito et al. (1992) reported an improved modification of the neutralization (NT) test for Inoue-Melnick Virus (IMV) by prolonged 90 min incubation of the mixture of the virus and serum. For confirmation, we compared the results of the NT test by 90 and 60 min incubation. Against human sera, the NT test by 90 min incubation was significantly more sensitive than the test by 60 min incubation. By 90 min incubation, the NT titers increased by 3 to 6 times and approximately 6% of antibody-negative adult sera previously determined in the tests by 60 min incubation turned to positive (more than 1:10). However, antibody-negative sera of Japanese children examined previously in the tests by 60 min incubation remained negative after the 90 min tests. On the other hand, against rabbit immune sera, no significant difference of NT titers was observed between the two NT tests for 90 and 60 min incubation. Also we reinvestigated Japanese adult sera for type 2 IMV infection by the NT test for 90 min incubation, but did not find any serum of type 2 or intermediate type infection. Therefore, it is not necessary to change the previous conclusion that in Japan IMV infections are mostly of type 1.
伊藤等人（1992年）报告称，通过将病毒与血清的混合物延长孵育90分钟，对井上-梅尔尼克病毒（IMV）的中和（NT）试验进行了改进。为了进行验证，我们比较了90分钟和60分钟孵育的NT试验结果。对于人血清，90分钟孵育的NT试验比60分钟孵育的试验明显更敏感。通过90分钟孵育，NT效价提高了3至6倍，并且在之前60分钟孵育试验中确定为抗体阴性的成年血清中，约6%变为阳性（大于1:10）。然而，之前在60分钟孵育试验中检测的日本儿童抗体阴性血清在90分钟试验后仍为阴性。另一方面，对于兔免疫血清，90分钟和60分钟孵育的两种NT试验之间未观察到NT效价有显著差异。我们还通过90分钟孵育的NT试验重新调查了日本成年血清中2型IMV感染情况，但未发现任何2型或中间型感染的血清。因此，没有必要改变之前的结论，即在日本IMV感染大多为1型。
