Kotrschal K, Whitear M, Finger T E
Konrad-Lorenz-Forschungsstelle für Ethologie, Grünau, Austria.
J Comp Neurol. 1993 May 15;331(3):407-17. doi: 10.1002/cne.903310310.
The pattern of innervation of the skin of the rockling Ciliata mustela was investigated to sort out spinal from facial nerve innervation of cutaneous chemosensory and mechanosensory systems. This fish has a variety of appendages with different functional sensory specializations, i.e., the chin barbel, pelvic fin, anterior dorsal fin, and dorsal trunk skin. The carbocyanine dye, diI, was applied to nerve stumps in dissected aldehyde-fixed tissue. In the case of the chin barbel, the dye was applied to both the trigeminal and facial nerve components. In the other cases, the dye was applied either selectively to the spinal nerves, to the facial nerves, or to both components. In the chin barbel, diI labeled nerve fibers associated with taste buds (TBs) and solitary chemosensory cells (SCCs) as well as relatively blunt free nerve endings, which closely approach the epidermal surface. In the pelvic fin, anterior dorsal fin, and dorsal trunk skin, taste buds, solitary chemosensory cells, and their innervation were labeled only after diI was applied to the facial nerve stumps. Application of diI to spinal nerves labeled delicate, free nerve endings and nerve fibers associated with small cells deep in the epidermis with features characteristic of Merkel cells. Transmission electron microscopy supports these results; after denervation of the facial component of the anterior dorsal fin, synaptic contacts with Merkel cells remained intact, whereas the synapses with the SCCs vanished.
为了区分纤唇鼬鳚皮肤化学感觉和机械感觉系统的脊髓神经与面神经支配，对纤唇鼬鳚（Ciliata mustela）皮肤的神经支配模式进行了研究。这种鱼有多种具有不同功能感觉特化的附属器官，即颏须、腹鳍、前背鳍和背部躯干皮肤。将羰花青染料二碘甲磺酸钠（diI）应用于解剖后的醛固定组织中的神经残端。对于颏须，将染料应用于三叉神经和面神经成分。在其他情况下，染料要么选择性地应用于脊神经、面神经，要么应用于两者。在颏须中，diI标记了与味蕾（TBs）和单个化学感觉细胞（SCCs）相关的神经纤维，以及相对钝圆的游离神经末梢，这些神经末梢靠近表皮表面。在腹鳍、前背鳍和背部躯干皮肤中，只有在将diI应用于面神经残端后，味蕾、单个化学感觉细胞及其神经支配才被标记。将diI应用于脊神经标记了精致的游离神经末梢以及与表皮深处具有默克尔细胞特征的小细胞相关的神经纤维。透射电子显微镜证实了这些结果；在前背鳍面神经成分去神经后，与默克尔细胞的突触接触保持完整，而与SCCs的突触消失。
