Kintz P, Mangin P
Forensic Scientists, Institut de Médecine Légale, Strasbourg-France.
J Forensic Sci. 1993 May;38(3):657-62.
The concentrations of morphine and codeine were investigated in hair from the head, axillary and pubic regions obtained from 12 fatal heroin cases. Hair preparation involves a decontamination procedure in dichloromethane at 37 degrees C for 15 min, solubilization in sodium hydroxyde at 100 degrees C for 5 min, neutralization with hydrochloric acid and centrifugation. After extraction in chloroform-isopropanol-n-heptane (50:17:33; v/v) at pH 9.2, drugs were derivatized with BSTFA + 1% TMCS and separated on a 12-m BP-5 capillary column. Quantification was done by GC/MS using selected ion monitoring. The highest morphine concentrations were found in pubic hair (0.80 to 41.34 ng/mg), followed by hair of the head (0.62 to 27.10 ng/mg), and axillary hair (0.40 to 24.20 ng/mg). Codeine was also detected in all samples, and the codeine-to-morphine ratios ranged from 0.069 to 0.273. The differences observed in drug concentration in the 3 types of hair are discussed in the light of the existing literature.
对12例致命海洛因中毒病例的头部、腋窝及耻骨区毛发中的吗啡和可待因浓度进行了研究。毛发制备过程包括在37℃的二氯甲烷中进行15分钟的去污处理，在100℃的氢氧化钠中溶解5分钟，用盐酸中和并离心。在pH 9.2的氯仿-异丙醇-正庚烷（50:17:33；v/v）中萃取后，药物用BSTFA + 1% TMCS进行衍生化，并在12米的BP-5毛细管柱上分离。采用选择离子监测通过气相色谱/质谱法进行定量分析。耻骨区毛发中吗啡浓度最高（0.80至41.34纳克/毫克），其次是头部毛发（0.62至27.10纳克/毫克）和腋窝毛发（0.40至24.20纳克/毫克）。所有样本中均检测到可待因，可待因与吗啡的比率在0.069至0.273之间。根据现有文献对3种毛发中药物浓度的差异进行了讨论。
