碘化示踪剂在13,14-二氢-15-酮前列腺素Fα灵敏放射免疫分析中的应用。

The use of iodinated tracers for a sensitive radioimmunoassay of 13,14-dihydro-15-ketoprostaglandin Falpha.

作者信息

Sors H, Maclouf J, Pradelles P, Dray F

出版信息

Biochim Biophys Acta. 1977 Mar 25;486(3):553-64. doi: 10.1016/0005-2760(77)90105-9.

Abstract

Radioimmunoassay of 13,14-dihydro-15-ketoprostaglandin Falpha is reported using various 125I-labelled derivatives. The apparent association constants of the antiserum for iodinated tracers are higher than with the homologous hapten. In spite of this, the high specific activities of iodinated tracers (2000 Ci/mmol) allow a 3-fold increase in the sensitivity of the assay when compared with the tritiated derivative. Human plasma levels of 13,14-dihydro-15-ketoprostaglandin Falpha reported (25+/-6 pg/ml) are lower than those previously found by radioimmunoassay, and no sexual difference was found.

摘要

据报道，使用各种125I标记的衍生物对13,14-二氢-15-酮前列腺素Fα进行放射免疫测定。抗血清对碘化示踪剂的表观结合常数高于对同源半抗原的结合常数。尽管如此，碘化示踪剂的高比活度（2000 Ci/mmol）使得该测定的灵敏度与氚化衍生物相比提高了3倍。所报道的人血浆中13,14-二氢-15-酮前列腺素Fα的水平（25±6 pg/ml）低于先前通过放射免疫测定法所发现的水平，且未发现性别差异。