Kovacs E J, Brock B, Silber I E, Neuman J E
Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Stritch School of Medicine, Maywood, Illinois.
Obstet Gynecol. 1993 Jul;82(1):29-36.
To assess the production of fibrogenic cytokines by interleukin-2 (IL-2)-stimulated peripheral blood leukocytes and to examine their ability to stimulate the production of connective tissue.
Culture medium from human peripheral blood leukocytes incubated with or without IL-2 was tested for induction of fibroblast proliferation, collagen synthesis, and expression of cytokine genes.
Supernatants from IL-2-treated peripheral blood leukocytes induced six times more fibroblast proliferation than medium from leukocytes cultured without IL-2. The expression of type I procollagen and fibronectin messenger RNAs was increased in human fibroblasts in response to leukocyte supernatants. Unstimulated leukocytes expressed minimal levels of transforming growth factor-beta or platelet-derived growth factor B chain messenger RNAs, but could be greatly enhanced by IL-2 treatment.
Mediators that induce connective tissue production are secreted by IL-2-treated peripheral blood leukocytes. These cytokines may be responsible, in part, for the stimulation of abdominal adhesions in patients receiving intraperitoneal immunotherapy.
评估白细胞介素-2（IL-2）刺激的外周血白细胞产生促纤维化细胞因子的情况，并检测其刺激结缔组织产生的能力。
检测与IL-2孵育或未孵育的人外周血白细胞的培养基，以诱导成纤维细胞增殖、胶原蛋白合成及细胞因子基因表达。
经IL-2处理的外周血白细胞的上清液诱导成纤维细胞增殖的能力比未用IL-2培养的白细胞培养基高五倍。I型前胶原和纤连蛋白信使核糖核酸的表达在人成纤维细胞中因白细胞上清液而增加。未受刺激的白细胞表达极低水平的转化生长因子-β或血小板衍生生长因子B链信使核糖核酸，但经IL-2处理后可大幅增强。
诱导结缔组织产生的介质由经IL-2处理的外周血白细胞分泌。这些细胞因子可能部分导致接受腹腔内免疫治疗的患者发生腹部粘连。
