Las Heras G, Millá F, Ribera J M, Oriol A, Feliu E
Servicio de Hematología y Hematerapia, Hospital Universitario Germans Trias i Pujol, Barcelona.
Sangre (Barc). 1993 Apr;38(2):97-102.
The aim of this study was to evaluate the haematologic autoanalyser System-9000 AX (Serono-Baker Diagnostics), which provides 15 parameters of red, white and platelet series and three histograms showing the cellular distribution curves according to the volume of each series.
The accuracy, precision, linearity, carry-over, effective speed and the effect of sample storage on results were studied. Moreover, the leucocyte differential count (LDC), false positive and negative results of the automated differential count and sensitivity, specificity and efficiency of LDC were analysed and compared with conventional methods: a Coulter VCS analyser and a Coulter S-Plus STKR.
The accuracy of the results of three haematologic series was very good. In the LDC, the results for the middle and big cells were better in comparison with the manual method (r > 0.80) than the Coulter VCS, (r = 0.28 and 0.48 respectively). The percentage of LDC revisions and the false negative was higher in hospitalized patients than in outpatients (28.14 and 8.46% versus 12.56 and 2.18%). The most common anomalies were the left deviation, monocytosis and eosinophilia. The results of sensitivity, specificity and efficiency were greater than 80% in all cases, except for the sensitivity and efficiency of hospitalized patients, because of the greater number of false negatives. Precision and linearity were excellent in all the parameters studied. No significant contamination was observed among the samples (low carry-over percentage). The samples were stable for 24 hours at 4 degrees C.
The System 9000 AX is a reliable analyser, which is fast and easy to operate. These characteristics allow this analyser to be a very useful tool for laboratories that require a rapid testing of samples.
本研究旨在评估血液自动分析仪System - 9000 AX（赛诺菲 - 贝克诊断公司），该分析仪可提供红细胞、白细胞和血小板系列的15项参数以及三个直方图，展示各系列细胞按体积分布的曲线。
研究了该分析仪的准确性、精密度、线性、携带污染、有效速度以及样本储存对结果的影响。此外，分析了白细胞分类计数（LDC）、自动分类计数的假阳性和假阴性结果以及LDC的敏感性、特异性和效率，并与传统方法进行比较：库尔特VCS分析仪和库尔特S - Plus STKR。
三个血液学系列结果的准确性非常好。在白细胞分类计数中，与手工方法相比，中型和大型细胞的结果更好（r > 0.80），优于库尔特VCS分析仪（分别为r = 0.28和0.48）。住院患者白细胞分类计数修正百分比和假阴性率高于门诊患者（分别为28.14%和8.46%，对比门诊患者的12.56%和2.18%）。最常见的异常是左移、单核细胞增多和嗜酸性粒细胞增多。除住院患者的敏感性和效率外，所有情况下敏感性、特异性和效率的结果均大于80%，原因是假阴性数量较多。在所研究的所有参数中，精密度和线性均极佳。样本间未观察到明显污染（携带污染百分比低）。样本在4℃下可稳定保存24小时。
System 9000 AX是一款可靠的分析仪，操作快速简便。这些特性使其成为需要快速检测样本的实验室的非常有用的工具。
