Hayashi T, Mukouyama M, Sakano K, Tani Y
Graduate School of Biological Sciences, Advanced Institute of Science and Technology, Nara, Japan.
Appl Environ Microbiol. 1993 May;59(5):1555-9. doi: 10.1128/aem.59.5.1555-1559.1993.
Arthrobacter sp. strain NO-18 was first isolated from soil as a bacterium which could degrade the sodium acrylate oligomer and utilize it as the sole source of carbon. When 0.2% (wt/wt) oligomer was added to the culture medium, the acrylate oligomer was found to be degraded by 70 to 80% in 2 weeks, using gel permeation chromatography. To determine the maximum molecular weight for biodegradation, the degradation test was done with the hexamer, heptamer, and octamer, which were separated from the oligomer mixture by fractional gel permeation chromatography. The hexamer and heptamer were consumed to the extents of 58 and 36%, respectively, in 2 weeks, but the octamer was not degraded. Oligomers with three different terminal groups were synthesized to examine the effect of the different terminal groups on biodegradation, but few differences were found. Arthrobacter sp. NO-18 assimilated acrylic acid, propionic acid, glutaric acid, 2-methylglutaric acid, and 1,3,5-pentanetricarboxylic acid. Degradation of the acrylic unit structure by this strain is discussed.
节杆菌属菌株NO-18最初是从土壤中分离出来的一种细菌，它能够降解丙烯酸钠低聚物并将其作为唯一碳源利用。当向培养基中添加0.2%（重量/重量）的低聚物时，使用凝胶渗透色谱法发现丙烯酸盐低聚物在2周内降解了70%至80%。为了确定生物降解的最大分子量，对通过分级凝胶渗透色谱法从低聚物混合物中分离出的六聚体、七聚体和八聚体进行了降解试验。六聚体和七聚体在2周内分别消耗了58%和36%，但八聚体未被降解。合成了具有三种不同端基的低聚物，以研究不同端基对生物降解的影响，但未发现明显差异。节杆菌属菌株NO-18同化了丙烯酸、丙酸、戊二酸、2-甲基戊二酸和1,3,5-戊三羧酸。讨论了该菌株对丙烯酸单元结构的降解情况。
