Knecht H, Joske D J, Bachmann E, Bachmann F, Odermatt B F, Pallesen G
Department of Internal Medicine, University Hospital CHUV Lausanne, Switzerland.
Br J Haematol. 1993 Apr;83(4):655-9. doi: 10.1111/j.1365-2141.1993.tb04706.x.
In order to determine the genotypic maturation status of the proliferating lymphoid cells in angioimmunoblastic lymphadenopathy (AILD) and in anaplastic large cell lymphoma of T-type (T-ALC), recombinase activating gene (RAG-1 and RAG-2) expression was assessed in six AILD and five T-ALC cases using a sensitive reverse transcriptase (RT) and competitive (C) polymerase chain reaction (PCR). RAG transcripts were not detectable in nine cases with high proliferating activity, suggesting that in most cases the proliferating cells are derived from mature (rearranged) lymphocytes. However, low levels of RAG transcripts were detected in one AILD and one T-ALC case and are consistent with either an involvement of immature lymphoid precursors in the proliferating pool or a deregulated T-cell maturation pathway with persistence of RAG expression. An association between RAG gene expression and poor response to therapy is possible but has to be tested in larger prospective series.
为了确定血管免疫母细胞性淋巴结病（AILD）和T型间变性大细胞淋巴瘤（T-ALC）中增殖性淋巴细胞的基因型成熟状态，我们使用灵敏的逆转录酶（RT）和竞争性（C）聚合酶链反应（PCR），对6例AILD和5例T-ALC病例的重组激活基因（RAG-1和RAG-2）表达进行了评估。在9例具有高增殖活性的病例中未检测到RAG转录本，这表明在大多数情况下，增殖细胞来源于成熟（重排）淋巴细胞。然而，在1例AILD和1例T-ALC病例中检测到低水平的RAG转录本，这与未成熟淋巴前体细胞参与增殖池或RAG表达持续存在的T细胞成熟途径失调是一致的。RAG基因表达与治疗反应不佳之间可能存在关联，但必须在更大的前瞻性系列研究中进行验证。
