Betz P, Nerlich A, Wilske J, Tübel J, Penning R, Eisenmenger W
Department of Legal Medicine, University of Munich, Germany.
Int J Legal Med. 1993;105(6):329-32. doi: 10.1007/BF01222117.
Collagen type III and V were visualized immunohistochemically in 79 surgically treated human skin wounds with a wound age between 8 h and 2.5 months. Network-like structures positively staining for collagen type III and associated with fibroblastic cells in the wound area were first detectable in a 2.5-day-old skin lesion and occurred regularly in wounds more than 5 days old. Collagen type V appeared first in the wound area after about 3 days, slightly later than collagen type III, and was detectable regularly in wounds with a survival time of 6 days or more. The immunohistochemical detection of collagen type III or type V thus indicates a wound age of at least 2-3 days. The lack of a positive reaction in a sufficient number of specimens indicates a wound age of less than 6 days. Even though both collagen types could also be detected in older wounds (wound age 2.5 months), further information for the time-estimation of older skin wounds cannot be given due to the observation that the time period during which reparative processes can be observed depends on the extent of the wound area.
对79例手术治疗的人类皮肤伤口进行免疫组织化学观察，伤口年龄在8小时至2.5个月之间。在2.5日龄的皮肤损伤中首次检测到III型胶原蛋白呈阳性染色的网络状结构，并与伤口区域的成纤维细胞相关，且在5日龄以上的伤口中经常出现。V型胶原蛋白在大约3天后首次出现在伤口区域，略晚于III型胶原蛋白，并且在存活时间为6天或更长时间的伤口中经常可检测到。因此，III型或V型胶原蛋白的免疫组织化学检测表明伤口年龄至少为2 - 3天。在足够数量的标本中缺乏阳性反应表明伤口年龄小于6天。尽管在较老的伤口（伤口年龄2.5个月）中也可以检测到这两种胶原蛋白类型，但由于观察到可观察到修复过程的时间段取决于伤口面积的大小，因此无法提供关于较老皮肤伤口时间估计的进一步信息。
