Betz P, Nerlich A, Tübel J, Penning R, Eisenmenger W
Department of Legal Medicine, University of Munich, Germany.
Int J Legal Med. 1993;105(4):229-32. doi: 10.1007/BF01642799.
The time-dependent reepithelialization of 55 human surgical skin wounds with a wound age between 8 h and more than 2 months was investigated by the immunohistochemical localization of cytokeratins 5 and 13. A complete, rebuilt epidermal layer over the wound area was first detectable in a 5-day-old wound, while all wounds of more than 18 days duration contained a completely reepithelialized wound area. Between 5 and 18 days the basal layer of keratinocytes showed--in contrast to normal skin--only some cells positive for cytokeratin 5. In some, but not all lesions with a wound age of 13 days or more, a basal cell layer completely staining for cytokeratin 5 was demonstrable. This staining pattern was found in all skin wounds with a wound age of more than 23 days. The immunohistochemical detection of cytokeratin 13 which can be observed regularly in non-cornifying squamous epithelia provides no information for the time-estimation of human skin wounds, since no significant temporary expression of this polypeptide seems to occur during the healing of human skin wounds.
通过细胞角蛋白5和13的免疫组织化学定位，对55例伤口年龄在8小时至2个月以上的人类外科皮肤伤口的时间依赖性再上皮化进行了研究。在5日龄的伤口中首次可检测到伤口区域上方完整的重建表皮层，而所有持续时间超过18天的伤口都有完全再上皮化的伤口区域。在5至18天之间，角质形成细胞的基底层与正常皮肤相比，仅显示一些细胞角蛋白5阳性。在一些（但不是所有）伤口年龄为13天或以上的病变中，可证明有一个完全被细胞角蛋白5染色的基底细胞层。在所有伤口年龄超过23天的皮肤伤口中都发现了这种染色模式。细胞角蛋白13在非角化鳞状上皮中可定期观察到，其免疫组织化学检测对人类皮肤伤口的时间估计没有提供信息，因为在人类皮肤伤口愈合过程中似乎没有这种多肽的明显短暂表达。
