Amorim C Z, Cordeiro R S, Vargaftig B B
Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Departamento de Fisiologia e Farmacodinâmica, Rio de Janeiro, Brazil.
Eur J Pharmacol. 1993 Apr 22;235(1):17-22. doi: 10.1016/0014-2999(93)90814-x.
The interference of the platelet-activating factor (PAF) receptor antagonist compound, WEB 2170, on death caused by antigen in boosted or unboosted immunized mice was investigated. Death was triggered by the i.v. injection of ovalbumin into animals actively sensitized 14 or 21 days before and that received (boosted) or did not receive (unboosted), a second immunization 14 days later. No significant difference in the response to PAF (50 micrograms/kg) or to ovalbumin (500 micrograms/kg) was noted in boosted or unboosted mice in terms of mortality. WEB 2170 was equieffective to prevent death by PAF in non-sensitized or sensitized boosted or unboosted mice. The i.p. treatment with WEB 2170 (8-16 mg/kg) 1 h before the antigenic challenge prevented death due to antigen in unboosted or in boosted mice. Our results suggest that PAF is involved in the anaphylactic shock in unboosted and boosted mice. In addition, different from the anaphylactic reaction developed in the mouse paw, the participation of PAF in the anaphylactic shock caused by antigen is not dependent on the delivery of a booster injection.
研究了血小板活化因子（PAF）受体拮抗剂化合物WEB 2170对加强免疫或未加强免疫小鼠抗原所致死亡的干扰作用。通过静脉注射卵清蛋白引发死亡，实验动物在14或21天前主动致敏，14天后接受（加强免疫）或未接受（未加强免疫）第二次免疫。就死亡率而言，加强免疫或未加强免疫的小鼠对PAF（50微克/千克）或卵清蛋白（500微克/千克）的反应无显著差异。在未致敏或致敏的加强免疫或未加强免疫小鼠中，WEB 2170预防PAF所致死亡的效果相同。在抗原攻击前1小时腹腔注射WEB 2170（8 - 16毫克/千克）可预防未加强免疫或加强免疫小鼠因抗原所致的死亡。我们的结果表明，PAF参与了未加强免疫和加强免疫小鼠的过敏性休克。此外，与小鼠爪部发生的过敏反应不同，PAF参与抗原所致过敏性休克并不依赖于加强注射。
