用于检测犬瘟热病毒的夹心斑点酶联免疫吸附测定法。
Sandwich-dot enzyme-linked immunosorbent assay for the detection of canine distemper virus.
作者信息
Li Zhi, Zhang Yanlong, Wang Huiguo, Jin Jinhua, Li Wenzhe
机构信息
Clinical Laboratory, Dalian Municipal Central Hospital, 826-Xinan Road, Shahekou District, Dalian city, Liaoning 116033, China (Zhi Li); Department of Wildlife Medicine, College of Wildlife Resources, Northeast Forestry University, 26-Hexing Road, Harbin, Heilongjiang 150040, China (Zhang); Institute of Immunology, College of Life Science and Technology, Dalian University, 10-Xuefu Avenue, Dalian Economical and Technological Development Zone, Liaoning 116622, China (Wang, Jin, Wenzhe Li).
出版信息
Can J Vet Res. 2013 Oct;77(4):303-8.
A sandwich-dot enzyme-linked immunosorbent assay (dot ELISA) was developed for the detection of canine distemper virus (CDV). In 56 dogs suspected to have CD the rates of detection of CDV antigen in samples of blood lymphocytes and palpebral conjunctiva by dot ELISA and ELISA were, respectively, 91% (49/54) and 81% (44/54) for the lymphocyte samples and 88% (28/32) and 75% (24/32) for the conjunctival samples. The CDV detection limits were 10 ng/50 μL for dot ELISA and 40 ng/50 μL for ELISA. The reliability of dot ELISA relative to electron microscopy was 96% with 22 samples: all 21 samples in which CDV particles were observed by electron microscopy yielded positive results with dot ELISA; the single sample in which particles were not observed yielded false-positive results with dot ELISA. The results indicate that the dot ELISA developed can serve as a reliable rapid diagnostic test in suspected cases of CD and also be useful for epidemiologic surveillance of the disease.
开发了一种夹心斑点酶联免疫吸附测定法（斑点酶联免疫吸附测定）用于检测犬瘟热病毒（CDV）。在56只疑似患有犬瘟热的犬中，通过斑点酶联免疫吸附测定和酶联免疫吸附测定法检测血液淋巴细胞和睑结膜样本中CDV抗原的阳性率，淋巴细胞样本分别为91%（49/54）和81%（44/54），结膜样本分别为88%（28/32）和75%（24/32）。斑点酶联免疫吸附测定法的CDV检测限为10 ng/50 μL，酶联免疫吸附测定法为40 ng/50 μL。对于22个样本，斑点酶联免疫吸附测定法相对于电子显微镜检查的可靠性为96%：通过电子显微镜观察到CDV颗粒的所有21个样本，斑点酶联免疫吸附测定法均呈阳性结果；未观察到颗粒的单个样本，斑点酶联免疫吸附测定法产生假阳性结果。结果表明，所开发的斑点酶联免疫吸附测定法可作为疑似犬瘟热病例的可靠快速诊断试验，也可用于该疾病的流行病学监测。
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