Gemma T, Iwatsuki K, Shin Y S, Yoshida E, Kai C, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
J Vet Med Sci. 1996 Aug;58(8):791-4. doi: 10.1292/jvms.58.791.
As a rapid and sensitive method to detect canine distemper virus (CDV), an immunocapture enzyme-linked immunosorbent assay (ELISA) was performed. The sensitivity and specificity of the immunocapture ELISA were considered to be high enough. Virus neutralizing (VN) test was also established using the immunocapture ELISA. By using this test, the different cross VN titers between sera of dogs experimentally infected with the Onderstepoort strain and those with a field isolate of CDV were observed.
作为一种检测犬瘟热病毒（CDV）的快速灵敏方法，进行了免疫捕获酶联免疫吸附测定（ELISA）。免疫捕获ELISA的灵敏度和特异性被认为足够高。还利用免疫捕获ELISA建立了病毒中和（VN）试验。通过使用该试验，观察到用 Onderstepoort 株实验感染的犬血清与感染犬瘟热病毒野外分离株的犬血清之间不同的交叉VN滴度。
