Kitsugi T, Yamamuro T, Nakamura T, Oka M
Department of Orthopaedic Surgery, Faculty of Medicine, Kyoto University, Japan.
Biomaterials. 1995 Sep;16(14):1101-7. doi: 10.1016/0142-9612(95)98907-v.
Four kinds of calcium phosphate ceramics, beta-calcium pyrophosphate (Ca2P2O7), beta-tricalcium phosphate (Ca3(PO4)2), hydroxyapatite (Ca10(PO4)6(OH)2) and tetracalcium phosphate (Ca4(PO4)2O), were prepared. The calcium/phosphorus molar ratios were 1, 1.5, 1.66 and 2, respectively. Particles (150-300 microns) of these ceramics were packed into holes (diameter 2.5 mm) made in the tibial metaphysis of mature male rats. At 2 weeks, 4 weeks, 8 weeks and 6 months after the operation, undecalcified specimens were prepared. Transmission electron microscopy showed that the bone-bonding behaviour of calcium phosphate ceramics at the interface with bone did not vary with the calcium/phosphate molar ratio. Amorphous substances or needle-like microcrystals were observed on the surface of the ceramics at 2 weeks after implantation. The ceramics showed direct continuity with small crystallites of bone tissue at 4 weeks, 8 weeks and 6 months after implantation. The ceramics appeared to be getting smaller with time. Collagen fibres were not observed at the bone/ceramic interface. Neither chemical bonding nor mechanical bonding by interlocking between bone and ceramics was described by morphological observation using transmission electron microscopy.
制备了四种磷酸钙陶瓷，即焦磷酸钙（Ca2P2O7）、β-磷酸三钙（Ca3(PO4)2）、羟基磷灰石（Ca10(PO4)6(OH)2）和磷酸四钙（Ca4(PO4)2O）。钙/磷摩尔比分别为1、1.5、1.66和2。将这些陶瓷的颗粒（150 - 300微米）填充到成熟雄性大鼠胫骨干骺端制作的孔（直径2.5毫米）中。在术后2周、4周、8周和6个月时，制备未脱钙标本。透射电子显微镜显示，磷酸钙陶瓷与骨界面处的骨结合行为并不随钙/磷摩尔比而变化。植入后2周时，在陶瓷表面观察到无定形物质或针状微晶。植入后4周、8周和6个月时，陶瓷与骨组织的小晶体呈现直接连续性。陶瓷似乎随着时间推移而变小。在骨/陶瓷界面未观察到胶原纤维。通过透射电子显微镜进行的形态学观察未描述骨与陶瓷之间的化学键合或机械互锁结合。
