不同成分磷酸钙骨移植材料中BMP-2递送对骨再生的影响。

Effects of BMP-2 Delivery in Calcium Phosphate Bone Graft Materials with Different Compositions on Bone Regeneration.

作者信息

Park Jin-Chul, Bae Eun-Bin, Kim Se-Eun, Kim So-Yun, Choi Kyung-Hee, Choi Jae-Won, Bae Ji-Hyeon, Ryu Jae-Jun, Huh Jung-Bo

机构信息

Department of Dentistry, School of Medicine, Korea University, Seoul 02841, Korea.

Department of Prosthodontics, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan 50612, Korea.

出版信息

Materials (Basel). 2016 Nov 23;9(11):954. doi: 10.3390/ma9110954.

This study was undertaken to investigate the effect of loading rhBMP-2 onto biphasic calcium phosphate (BCP) and calcium pyrophosphate (CPP) on bone regeneration, and to examine the efficacies of BCP and CPP as rhBMP-2 carriers. Specimens were divided into the BCP, CPP, BCP/BMP, and CPP/BMP groups; BCP and CPP were in granules and not coated with rhBMP-2. BCP/BMP and CPP/BMP were prepared as discs, which were treated with rhBMP-2 and collagen. Physical and biological features were investigated using in-vitro and in-vivo tests. New bone area percentages (%) in the BCP/BMP and CPP/BMP groups were significantly greater than in the BCP and CPP groups. At weeks 4 and 8 post-implantation, CPP/BMP showed the most new bone growth. Within the limitations of this study, treatment of BCP and CPP with rhBMP-2 significantly enhanced bone regeneration. CPP was found to be a suitable carrier for rhBMP-2.

本研究旨在探讨将重组人骨形态发生蛋白-2（rhBMP-2）负载于双相磷酸钙（BCP）和焦磷酸钙（CPP）上对骨再生的影响，并考察BCP和CPP作为rhBMP-2载体的效果。将标本分为BCP组、CPP组、BCP/BMP组和CPP/BMP组；BCP和CPP为颗粒状，未包被rhBMP-2。BCP/BMP和CPP/BMP制成圆盘状，用rhBMP-2和胶原蛋白处理。通过体外和体内试验研究其物理和生物学特性。BCP/BMP组和CPP/BMP组的新骨面积百分比（%）显著高于BCP组和CPP组。植入后第4周和第8周，CPP/BMP显示出最多的新骨生长。在本研究的局限性范围内，用rhBMP-2处理BCP和CPP可显著增强骨再生。发现CPP是rhBMP-2的合适载体。