黑曲霉内切-β-1,4-D-半乳聚糖酶的纯化及性质
Purification and properties of endo-beta-1,4-D-galactanase from Aspergillus niger.
作者信息
Yamaguchi F, Inoue S, Hatanaka C
机构信息
Food R & D Center, Japan Tobacco Inc., Kanagawa, Japan.
出版信息
Biosci Biotechnol Biochem. 1995 Sep;59(9):1742-4. doi: 10.1271/bbb.59.1742.
PMID:8520117
Abstract
An endo-beta-1,4-D-galactanase was purified 1735-fold from a commercial enzyme preparation of Aspergillus niger. The endogalactanase was homogeneous by SDS-PAGE, having an apparent molecular weight of 32,000. It specifically hydrolyzed beta-1,4-D-galactan, and is shown to be capable of releasing galactosyl oligomers and galactose from the soybean pectic polysaccharides.
摘要
从黑曲霉的一种商业酶制剂中纯化出一种内切-β-1,4-D-半乳聚糖酶，纯化倍数为1735倍。该内切半乳聚糖酶经SDS-PAGE分析显示为均一的，表观分子量为32000。它能特异性水解β-1,4-D-半乳聚糖，并且能够从大豆果胶多糖中释放出半乳糖基低聚物和半乳糖。
Zotero 插件