Asakura T, Abe K, Arai S
Laboratory for Food Science, Atomi Junior College, Tokyo, Japan.
Biosci Biotechnol Biochem. 1995 Sep;59(9):1793-4. doi: 10.1271/bbb.59.1793.
Using reverse transcription polymerase chain reaction (RT-PCR), we identified two cDNA fragments encoding aspartic proteinases (APs). The encoded proteins, pL4 and pL5, are similar in sequence to other plant APs including barley AP and cardoon flower AP (cyprosin). The identity was 58-76% with one another. Genomic Southern blot analysis suggested that the rice AP gene constitutes a multigene family.
利用逆转录聚合酶链反应（RT-PCR），我们鉴定出了两个编码天冬氨酸蛋白酶（APs）的cDNA片段。所编码的蛋白pL4和pL5在序列上与其他植物天冬氨酸蛋白酶相似，包括大麦天冬氨酸蛋白酶和刺菜蓟花天冬氨酸蛋白酶（环孢菌素）。它们之间的一致性为58%-76%。基因组Southern杂交分析表明，水稻天冬氨酸蛋白酶基因构成了一个多基因家族。
