Machado M C, Coelho A M, Kubrusly M S, Bonizzia A, Pinotti H W
Departamento de Gastroenterologia, Faculdade de Medicina, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1995 Apr;28(4):471-5.
A previous report has shown that undernutrition reduces the mortality of acute experimental pancreatitis probably by decreasing pancreatic enzyme content. Cerulein in physiological doses reduces the enzyme content of the pancreas without any harmful effect on the organ. The aim of the present study was to asses the effect of acute reduction of pancreatic enzyme content on the outcome of acute pancreatitis. Two groups of male Wistar rats weighing 230-250 g were studied: group I, 12-h fasted animals, and group II, ad libitum-fed animals who received cerulein at the inframaximal dose (0.2 microgram kg-1 h-1) for 2 h. Cerulein administration resulted in the reduction of the pancreatic contents of chymotrypsinogen (71%), trypsinogen (55%), proelastase (60%), amylase (62%) and cathepsin B (45%) (P < 0.05). However, no significant reduction in pancreatic phospholipase content was observed. Acute pancreatitis was induced in group I after 12-h fasting and in group II at the end of cerulein infusion by retrograde injection o 0.5 ml of 2.5% Na+ taurocholate into the pancreatic duct. Ascites volume and the degree of histologically observed lesions were similar in both groups, but 72-h mortality was 56% in the control group (10/ 18) and 23% (5/22) in the cerulein group (P < 0.05). We speculate that the reduction of pancreatic enzyme content may exert its beneficial effect in acute pancreatitis by decreasing the quantity of pancreatic enzymes reaching the circulation and consequently their pathogenic effects.
先前的一份报告显示，营养不良可能通过降低胰腺酶含量来降低急性实验性胰腺炎的死亡率。生理剂量的雨蛙肽可降低胰腺的酶含量，而对该器官没有任何有害影响。本研究的目的是评估急性降低胰腺酶含量对急性胰腺炎预后的影响。研究了两组体重为230 - 250克的雄性Wistar大鼠：第一组为禁食12小时的动物，第二组为自由进食的动物，它们接受次最大剂量（0.2微克·千克⁻¹·小时⁻¹）的雨蛙肽注射2小时。注射雨蛙肽导致胰凝乳蛋白酶原（71%）、胰蛋白酶原（55%）、弹性蛋白酶原（60%）、淀粉酶（62%）和组织蛋白酶B（45%）的胰腺含量降低（P < 0.05）。然而，未观察到胰腺磷脂酶含量有显著降低。第一组在禁食12小时后诱导急性胰腺炎，第二组在雨蛙肽输注结束时通过向胰管逆行注射0.5毫升2.5%的牛磺胆酸钠诱导急性胰腺炎。两组的腹水量和组织学观察到的损伤程度相似，但对照组的72小时死亡率为56%（10/18），雨蛙肽组为23%（5/22）（P < 0.05）。我们推测，胰腺酶含量的降低可能通过减少进入循环的胰腺酶数量从而降低其致病作用，在急性胰腺炎中发挥有益作用。
