Chauvin A, L'Hostis M, Valentin A, Precigout E, Cesbron-Zeggane N, Gorenflot A
Laboratoire de Parasitologie, Ecole Nationale Vétérinaire de Nantes.
Parasite. 1995 Sep;2(3):257-62. doi: 10.1051/parasite/1995023257.
An enzyme linked immunosorbent assay (ELISA) for bovine babesiosis caused by Babesia divergens was developed to analyse the evolution of the serological status of cattle living in an enzootic area. The antigen used was a soluble extract of B. divergens obtained from in vitro culture. Specificity, evaluated with negative sera, was 96.6%. The ELISA was compared to indirect immunofluorescence analysis (IFA) on naturally or experimentally infected animals. It appeared that IFA was positive for at least seven or eight weeks; on the contrary, B. divergens-specific antibodies were only transitorily detected by ELISA. Furthermore, the ELISA was more sensitive than the IFA for the detection of post-infection antibody increases, particularly when infection-pressure was low. These results suggest that IFA and ELISA could be complementary tools in epidemiological surveys; indeed, this ELISA could be the most efficient tool to detect new infections in cohort monitoring.
开发了一种用于检测由分歧巴贝斯虫引起的牛巴贝斯虫病的酶联免疫吸附测定（ELISA），以分析生活在地方病流行区的牛的血清学状态演变。所用抗原是从体外培养物中获得的分歧巴贝斯虫可溶性提取物。用阴性血清评估的特异性为96.6%。将ELISA与自然感染或实验感染动物的间接免疫荧光分析（IFA）进行比较。结果显示，IFA至少在七至八周内呈阳性；相反，ELISA仅能短暂检测到分歧巴贝斯虫特异性抗体。此外，在检测感染后抗体增加方面，ELISA比IFA更敏感，尤其是在感染压力较低时。这些结果表明，IFA和ELISA在流行病学调查中可能是互补工具；实际上，这种ELISA可能是队列监测中检测新感染的最有效工具。
