基于聚合酶链反应（PCR）的重叠延伸法进行精确的大片段缺失
Precise large deletions by the PCR-based overlap extension method.
作者信息
Senanayake S D, Brian D A
机构信息
Department of Microbiology, University of Tennessee, Knoxville 37996-0845, USA.
出版信息
Mol Biotechnol. 1995 Aug;4(1):13-5. doi: 10.1007/BF02907467.
Abstract
The authors describe an efficient method for generating large deletions (> 200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.
摘要
作者描述了一种利用基于聚合酶链式反应（PCR）的重叠延伸基因剪接方法（1）来产生精确长度的大片段缺失（>200个核苷酸）的高效方法。该方法在技术上简单，且比需要制备单链DNA模板的传统环出诱变技术耗时更少。
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