一种用于定量测定血液中奎尼丁的气液色谱法。

A gas-liquid chromatographic method for the quantitative determination of quinidine in blood.

作者信息

Moulin M A, Kinsun H

出版信息

Clin Chim Acta. 1977 Mar 15;75(3):491-5. doi: 10.1016/0009-8981(77)90371-0.

Abstract

A gas-liquid chromatographic method, using chloroquine as internal standard, for quinidine evaluation in serum is described. Prior to acidic delipidation, chloroquine is added to the serum sample and an alkaline extraction is used. Direct gas-liquid chromatographic analysis of unaltered forms using a nitrogen detector is performed. The sensitivity limit of this method is 0.5 microng/ml, i.e. sufficient for therapeutic quinidine blood level determinatin (1 to 5 microng/ml).

摘要

描述了一种以氯喹为内标物的气-液色谱法，用于血清中奎尼丁的评估。在进行酸性脱脂之前，向血清样品中加入氯喹，并采用碱性萃取。使用氮检测器对未改变的形式进行直接气-液色谱分析。该方法的检测限为0.5微克/毫升，即足以用于治疗性奎尼丁血药浓度的测定（1至5微克/毫升）。