V(D)J重组信号的切割仅需RAG1和RAG2蛋白，并分两步进行。

Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps.

作者信息

McBlane J F, van Gent D C, Ramsden D A, Romeo C, Cuomo C A, Gellert M, Oettinger M A

机构信息

Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0540, USA.

出版信息

Cell. 1995 Nov 3;83(3):387-95. doi: 10.1016/0092-8674(95)90116-7.

DOI:10.1016/0092-8674(95)90116-7

PMID:8521468

Abstract

Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAG2 proteins are sufficient to carry out this reaction. The cleavage reaction can be divided into two distinct steps. First, a nick is introduced at the 5' end of the signal sequence. The other strand is then broken, resulting in a hairpin structure at the coding end and a blunt, 5'-phosphorylated signal end. The hairpin is made as a direct consequence of the cleavage mechanism. Nicking and hairpin formation each require the presence of a signal sequence and both RAG proteins.

摘要

在重组信号序列处形成双链断裂是V(D)J重组的早期步骤。我们在此表明，纯化的RAG1和RAG2蛋白足以进行此反应。切割反应可分为两个不同的步骤。首先，在信号序列的5'端引入一个切口。然后另一条链被切断，导致编码端形成发夹结构和钝性的、5'-磷酸化的信号端。发夹结构是切割机制的直接结果。切口形成和发夹结构形成均需要信号序列和两种RAG蛋白的存在。