Kumar R, Kumar R
Department of Veterinary Parasitology, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar, India.
Exp Appl Acarol. 1995 Jun;19(6):319-24. doi: 10.1007/BF00052389.
New Zealand white rabbits were immunized with partly fed Hyalomma dromedarii tick-derived midgut concealed antigens (supernate and pellet fractions) and Freund's complete adjuvant (FCA). The rabbits received three inoculations subcutaneously on days 0, 14 and 21 at a dose rate of 1 mg antigen per animal. The effects of the immunity induced was determined by infesting the rabbits with adult H. dromedarii ticks. In immunized rabbits a significant reduction in tick yield, engorgement weight, oviposition period, egg mass weight and percentage of egg hatchability was found. The gut supernatant antigen fraction induced the best protection in terms of reduced feeding and reproductive performance of the ticks.
用部分喂食的单峰驼璃眼蜱中肠隐蔽抗原（上清液和沉淀组分）与弗氏完全佐剂（FCA）对新西兰白兔进行免疫。兔子在第0、14和21天接受三次皮下接种，每只动物的抗原剂量率为1毫克。通过用成年单峰驼璃眼蜱感染兔子来确定诱导免疫的效果。在免疫的兔子中，发现蜱的产量、饱血重量、产卵期、卵块重量和卵孵化率百分比显著降低。就蜱的取食和繁殖性能降低而言，肠道上清液抗原组分诱导出了最佳的保护效果。
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