El Hakim Amr E, Shahein Yasser E, Abdel-Shafy Sobhy, Abouelella Amira Mk, Hamed Ragaa R
Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt.
J Vet Sci. 2011 Sep;12(3):243-9. doi: 10.4142/jvs.2011.12.3.243.
In order to identify antigens that can help prevent camel tick infestations, three major glycoproteins (GLPs) about 97, 66 and 40 kDa in size were purified from adult and larval Egyptian ticks, Hyalomma (H.) dromedarii, using a single-step purification method with Con-A sepharose. The purified GLPs were evaluated as vaccines against camel tick infestation in rabbits. The rabbits received three intramuscular inoculations of GLPs (20 µg/animal) on days 0, 14, and 28. In the immunoblot analysis, Sera from the immunized rabbits recognized the native GLPs and other proteins from larval and adult H. dromedarii ticks along with those from other tick species such as Rhipicephalus sanguineus but not Ornithodoros moubata. The effects of immunity induced by these GLPs were determined by exposing rabbits to adult H. dromedarii ticks. These results demonstrated that GLP immunization led to a slightly decreased reproductive index and significantly reduced rates of egg hatchability. These results demonstrated that immunization with the purified GLPs can provide protection against infestation by H. dromedarii and some other tick species. Further studies are needed to confirm the effectiveness of immunization with GLPs against other tick species.
为了鉴定有助于预防骆驼蜱虫侵扰的抗原，采用伴刀豆球蛋白A琼脂糖一步纯化法，从成年和幼虫埃及单峰驼璃眼蜱（Hyalomma (H.) dromedarii）中纯化出三种主要糖蛋白（GLPs），大小分别约为97、66和40 kDa。将纯化的GLPs作为抗骆驼蜱虫侵扰的疫苗在兔子身上进行评估。兔子在第0、14和28天接受三次GLPs的肌肉注射（20 µg/只动物）。在免疫印迹分析中，免疫兔子的血清识别天然GLPs以及来自幼虫和成年单峰驼璃眼蜱的其他蛋白质，还有来自其他蜱种（如血红扇头蜱）的蛋白质，但不识别波斯锐缘蜱。通过让兔子接触成年单峰驼璃眼蜱来确定这些GLPs诱导的免疫效果。这些结果表明，GLP免疫导致生殖指数略有下降，卵孵化率显著降低。这些结果表明，用纯化的GLPs进行免疫可以提供针对单峰驼璃眼蜱和其他一些蜱种侵扰的保护。需要进一步研究来证实用GLPs免疫对其他蜱种的有效性。
