Sanne J L, Krueger K E
Fidia-Georgetown Institute for the Neurosciences, Georgetown University School of Medicine, Washington, DC 20007, USA.
Gene. 1995 Nov 20;165(2):327-8. doi: 10.1016/0378-1119(95)00536-f.
Polymerase chain reaction amplification of the cDNA encoding steroid 17 alpha-hydroxylase (P450c17) demonstrated very low levels of this transcript and a shorter variant of still lower abundance in rat adrenals and brain. Sequence analysis of the two amplified products revealed that the shorter variant resulted from a deletion of the second exon which does not maintain the open reading frame, suggesting that faulty splicing gave rise to this minor species.
对编码类固醇17α-羟化酶（P450c17）的cDNA进行聚合酶链反应扩增，结果显示在大鼠肾上腺和脑中该转录本水平极低，且存在一种丰度更低的较短变体。对这两种扩增产物进行序列分析发现，较短变体是由于第二个外显子缺失所致，该缺失破坏了开放阅读框，提示错误剪接产生了这种次要产物。
