McClean E, McGrath L T, Archbold G P
Department of Clinical Chemistry, Belfast City Hospital, N. Ireland.
Ir J Med Sci. 1995 Oct-Dec;164(4):289-92. doi: 10.1007/BF02967207.
Two fluorescent probes were used for the measurement of membrane fluidity in patients on haemodialysis and continuous ambulatory peritoneal dialysis. 1,6-Diphenyl-1,3,5-hexatriene (DPH) anisotropy gives an indication of lipid order and pyrene measures lateral diffusion through the membrane. Pyrene dimer/monomer ratio was significantly lower than controls in both pre-dialysis and post-dialysis samples but DPH anisotropy was unchanged. Both methods showed an increase in membrane fluidity across a 4 hour haemodialysis session. There was an increase in membrane fluidity in CAPD patient samples which was more marked using DPH than pyrene. These results suggest that the two probes give different but complementary information about changes in membrane fluidity and may be more informative when used together rather than singly.
使用两种荧光探针来测量接受血液透析和持续性非卧床腹膜透析的患者的膜流动性。1,6-二苯基-1,3,5-己三烯（DPH）各向异性反映脂质有序性，芘用于测量通过膜的横向扩散。在透析前和透析后的样本中，芘二聚体/单体比率均显著低于对照组，但DPH各向异性未发生变化。两种方法均显示在4小时的血液透析过程中膜流动性增加。持续性非卧床腹膜透析患者样本中的膜流动性增加，使用DPH检测时比使用芘检测时更明显。这些结果表明，这两种探针提供了关于膜流动性变化的不同但互补的信息，一起使用时可能比单独使用更具信息量。
