Saunders N J, Want S V, Adams D J
Department of Infectious Diseases and Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
J Antimicrob Chemother. 1995 Aug;36(2):411-5. doi: 10.1093/jac/36.2.411.
Serum with vancomycin concentrations between 5 and 15 mg/L from patients on dialysis were assayed by fluorescence polarization immunoassay (FPIA) and enzyme multiplied immunoassay technique (EMIT). The concentrations as determined by FPIA were higher than those using EMIT (mean difference 2.1 mg/L, S.D. 1.5; range -0.8-6.3) with substantial interpatient variability in the difference between the two assays. This suggests that concentrations of active vancomycin are lower than indicated by FPIA and thresholds for redosing may need to be adjusted.
采用荧光偏振免疫分析法（FPIA）和酶放大免疫分析技术（EMIT）对透析患者血清中万古霉素浓度在5至15mg/L之间的样本进行测定。FPIA测定的浓度高于EMIT测定的浓度（平均差值2.1mg/L，标准差1.5；范围-0.8至6.3），两种检测方法之间的差异在患者间存在很大变异性。这表明活性万古霉素的浓度低于FPIA所显示的浓度，可能需要调整再次给药的阈值。
