Kozminski K G, Beech P L, Rosenbaum J L
Department of Biology, Yale University, New Haven, Connecticut 06511, USA.
J Cell Biol. 1995 Dec;131(6 Pt 1):1517-27. doi: 10.1083/jcb.131.6.1517.
The Chlamydomonas FLA10 gene was shown to encode a flagellar kinesin-like protein (Walther, Z., M. Vashishtha, and J.L. Hall. 1994. J. Cell Biol. 126:175-188). By using a temperature-sensitive allele of FLA10, we have determined that the FLA10 protein is necessary for both the bidirectional movement of polystyrene beads on the flagellar membrane and intraflagellar transport (IFT), the bidirectional movement of granule-like particles beneath the flagellar membrane (Kozminski, K.G., K.A. Johnson, P. Forscher, and J.L. Rosenbaum. 1993. Proc. Natl. Acad. Sci. (USA). 90:5519-5523). In addition, we have correlated the presence and position of the IFT particles visualized by light microscopy with that of the electron dense complexes (rafts) observed beneath the flagellar membrane by electron microscopy. A role for FLA10 in submembranous or flagellar surface motility is also strongly supported by the immunolocalization of FLA10 to the region between the axonemal outer doublet microtubules and the flagellar membrane.
衣藻FLA10基因被证明编码一种鞭毛驱动蛋白样蛋白（Walther, Z., M. Vashishtha, and J.L. Hall. 1994. J. Cell Biol. 126:175 - 188）。通过使用FLA10的温度敏感等位基因，我们已经确定FLA10蛋白对于聚苯乙烯珠子在鞭毛膜上的双向移动以及鞭毛内运输（IFT）都是必需的，鞭毛内运输是指颗粒状颗粒在鞭毛膜下方的双向移动（Kozminski, K.G., K.A. Johnson, P. Forscher, and J.L. Rosenbaum. 1993. Proc. Natl. Acad. Sci. (USA). 90:5519 - 5523）。此外，我们已经将通过光学显微镜观察到的IFT颗粒的存在和位置与通过电子显微镜在鞭毛膜下方观察到的电子致密复合物（筏）的存在和位置关联起来。通过将FLA10免疫定位到轴丝外双联微管和鞭毛膜之间的区域也有力地支持了FLA10在膜下或鞭毛表面运动中的作用。
