Wilson P J, White B N
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
J Forensic Sci. 1998 May;43(3):477-82.
We have developed a PCR-based protocol to determine the gender of tissue samples originating from elk (Cervus elaphus canadensis), moose (Alces alces) and white-tailed deer (Odocoileus virginianus). The technique simultaneously amplifies a conserved region of the sex-determining gene on the Y-chromosome (Sry) and a region of the Fragile X mental retardation gene (Fmr-1). The multiplex nature of this protocol allows the determination of gender using the Sry marker with the Fmr-1 marker providing an internal control. This technique is applicable to the enforcement of the validation tag system for game species. Data are provided from a wildlife investigation in Ontario.
我们开发了一种基于聚合酶链反应（PCR）的方法，用于确定源自驼鹿（加拿大马鹿）、驼鹿（美洲驼鹿）和白尾鹿（弗吉尼亚鹿）的组织样本的性别。该技术同时扩增Y染色体上性别决定基因（Sry）的保守区域和脆性X智力障碍基因（Fmr-1）的一个区域。此方法的多重性质允许使用Sry标记来确定性别，而Fmr-1标记作为内部对照。该技术适用于实施野生动物物种的验证标签系统。文中提供了安大略省一项野生动物调查的数据。
