Ballmer P E, McNurlan M A, Grant I, Garlick P J
Rowett Research Institute, Aberdeen, United Kingdom.
JPEN J Parenter Enteral Nutr. 1995 Jul-Aug;19(4):266-71. doi: 10.1177/0148607195019004266.
Inflammatory stimuli and provision of nutrients are important factors regulating both total liver protein synthesis and albumin synthesis.
The influence of interleukin-1 beta or turpentine injection and a 2-hour infusion of a hypocaloric mixture of glucose and amino acids on the synthesis of total liver protein and albumin was investigated in rats. Total liver protein synthesis was measured by an i.v. flooding dose of L-[2,6(3)H]phenylalanine and albumin synthesis was determined from the labeling of immunoprecipitated albumin and expressed both as a fraction of total liver protein synthesis and as an absolute rate.
Interleukin-1 beta or turpentine injection stimulated total liver protein synthesis compared with controls, whereas albumin synthesis, both as a fraction of total liver protein synthesis and as an absolute synthesis rate, decreased. In control animals, the 2-hour i.v. infusion with glucose and amino acids resulted in a significant increase of total liver protein synthesis. Albumin synthesis as a fraction of total liver protein synthesis was not altered, but increased when expressed as an absolute rate in control animals. However, interleukin-1 beta or turpentine injection abolished this response of albumin synthesis to nutrient supply.
Total liver protein synthesis increases under inflammatory conditions and remains responsive to nutrient supply. In contrast, albumin synthesis decreases under the same conditions and does not seem to be responsive to short-term i.v. nutrients.
炎症刺激和营养物质供应是调节肝脏总蛋白合成和白蛋白合成的重要因素。
研究了白细胞介素-1β或松节油注射以及2小时输注葡萄糖和氨基酸的低热量混合物对大鼠肝脏总蛋白和白蛋白合成的影响。通过静脉注射大剂量L-[2,6(3)H]苯丙氨酸来测量肝脏总蛋白合成，从免疫沉淀白蛋白的标记中测定白蛋白合成，并以肝脏总蛋白合成的分数和绝对速率表示。
与对照组相比，白细胞介素-1β或松节油注射刺激了肝脏总蛋白合成，而白蛋白合成，无论是作为肝脏总蛋白合成的分数还是绝对合成速率，均下降。在对照动物中，2小时静脉输注葡萄糖和氨基酸导致肝脏总蛋白合成显著增加。白蛋白合成作为肝脏总蛋白合成的分数没有改变，但在对照动物中以绝对速率表示时增加。然而，白细胞介素-1β或松节油注射消除了白蛋白合成对营养供应的这种反应。
在炎症条件下肝脏总蛋白合成增加，并且仍然对营养供应有反应。相比之下，在相同条件下白蛋白合成减少，并且似乎对短期静脉营养无反应。
