Vasconcelos H C, Yoshida C F, Vanderborght B O, Schatzmayr H G
Centro de Hematologia e Hemoterapia de Santa Catarina-HEMOSC, Setor de Sorologia, Florianópolis, SC, Brasil.
Mem Inst Oswaldo Cruz. 1994 Oct-Dec;89(4):503-7. doi: 10.1590/s0074-02761994000400002.
The prevalence of hepatitis B and C infection has been determined in a seroepidemiological survey among blood donors from the south of Brazil (Florianópolis, State of Santa Catarina). These markers has also been correlated with the levels of alanine aminotransferase (ALT), a surrogate marker to prevent post-transfusion hepatitis. Sera from 5000 donors were randomly collected in the period of April to November 1991. The prevalences of HBsAg, anti-HBs and anti-HBc were respectively 0.78%, 7.02% and 13.98%. The anti-HCV prevalence after confirmation testing with line immunoassay (LIA), was 1.14%. Normal values of ALT ( < = 32 U/ml) were found in 59.78%, values slightly above the mean (ALT between 32-70 U/ml) in 37.74% and high values of ALT ( > = 70 U/ml) in 2.48%. The positivity of anti-HCV antibodies increased with the elevation of ALT levels. This correlation was not observed in relation to HBsAg. There exists a diversity in the recognition of HCV epitopes among HCV positive donors. Via the confirmation test used, we could observe that 94.7% of donors recognize the structural core antigen. Besides that, we observed that 5.26% of the HCV reactive sera recognized only epitopes located in the NS4 and/or NS5 region, indicating the importance of these epitopes for the improvement of assays.
在巴西南部（圣卡塔琳娜州弗洛里亚诺波利斯）对献血者进行的血清流行病学调查中，已确定了乙型和丙型肝炎感染的流行情况。这些标志物还与丙氨酸转氨酶（ALT）水平相关，ALT是预防输血后肝炎的替代标志物。1991年4月至11月期间，随机收集了5000名献血者的血清。HBsAg、抗-HBs和抗-HBc的流行率分别为0.78%、7.02%和13.98%。经线性免疫测定（LIA）确认检测后，抗-HCV流行率为1.14%。ALT正常（<=32 U/ml）的占59.78%，略高于平均值（ALT在32-70 U/ml之间）的占37.74%，ALT高值（>=70 U/ml）的占2.48%。抗-HCV抗体阳性率随ALT水平升高而增加。HBsAg未观察到这种相关性。HCV阳性献血者中对HCV表位的识别存在差异。通过所使用的确认试验，我们可以观察到94.7%的献血者识别结构核心抗原。除此之外，我们观察到5.26%的HCV反应性血清仅识别位于NS4和/或NS5区域的表位，这表明这些表位对改进检测方法的重要性。
